Mousavi Kouhi Seyed Mousa, Lahouti Mehrdad, Ganjeali Ali, Entezari Mohammad H
Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran,
Environ Sci Pollut Res Int. 2015 Jul;22(14):10733-43. doi: 10.1007/s11356-015-4306-0. Epub 2015 Mar 11.
Rapid development of nanotechnology in recent years has raised concerns about nanoparticle (NPs) release into the environment and its adverse effects on living organisms. The present study is the first comprehensive report on the anatomical and ultrastructural changes of a variety of cells after long-term exposure of plant to NPs or bulk material particles (BPs). Light and electron microscopy revealed some anatomical and ultrastructural modifications of the different types of cell in the root and leaf, induced by both types of treatment. Zinc oxide (ZnO) BPs-induced modifications were surprisingly more than those induced by ZnO NPs. The modifications induced by ZnO BPs or ZnO NPs were almost similar to those induced by excess Zn. Zn content of the root and leaf of both ZnO NPs- and ZnO BPs-treated plants was severely increased, where the increase was greater in the plants treated with ZnO BPs. Overall, these results indicate that the modifications induced by ZnO particles can be attributed, at least partly, to the Zn(2+) dissolution by ZnO particles rather than their absorption by root and their subsequent effects.
近年来纳米技术的快速发展引发了人们对纳米颗粒（NPs）释放到环境中及其对生物有机体产生不利影响的担忧。本研究是关于植物长期暴露于纳米颗粒或块状材料颗粒（BPs）后多种细胞的解剖学和超微结构变化的首份综合报告。光学显微镜和电子显微镜显示，两种处理方式均诱导了根和叶中不同类型细胞的一些解剖学和超微结构改变。令人惊讶的是，氧化锌（ZnO）块状颗粒诱导的改变比氧化锌纳米颗粒诱导的更多。ZnO块状颗粒或ZnO纳米颗粒诱导的改变与过量锌诱导的改变几乎相似。ZnO纳米颗粒处理组和ZnO块状颗粒处理组植物的根和叶中的锌含量均显著增加，其中ZnO块状颗粒处理组植物的增加幅度更大。总体而言，这些结果表明，ZnO颗粒诱导的改变至少部分可归因于ZnO颗粒的Zn(2+)溶解，而非其被根吸收及其后续影响。
