Kukreti Bharti, Sharma Anita, Chaudhary Parul, Agri Upasana, Maithani Damini
Department of Microbiology, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263145 India.
3 Biotech. 2020 Aug;10(8):345. doi: 10.1007/s13205-020-02329-8. Epub 2020 Jul 21.
Application of nanocompounds along with plant growth promoting rhizobacteria is gaining attention to improve agriculture productivity. In the present study, attempts have been made to observe the impact of nanosilicon dioxide (10 mg L) and two plant growth promotory bacteria (PC1-MK106029) and (PC4-MK106024) on the growth of and its rhizosphere in a pot experiment. Combined treatment of bacterial consortium and nanosilicon dioxide enhanced average plant height and number of leaves over control in maize after 30 days of sowing. Similarly, percent enhancement of total chlorophyll, carotenoid, sugar, soluble protein, phenol and flavonoid content was 106, 307, 116, 57, 159 and 132 respectively over control in maize leaves in the same treatment. Treated plants showed significant increase of 29.4 and 73.9% in catalase and peroxidase activities respectively over control. Physicochemical and biochemical parameters of soil health were also improved in the soil treated with PGPR and nanosilicon dioxide. An increase of 1.5-2 fold in the activities of fluorescein diacetate, dehydrogenase and alkaline phosphatase was observed in the treated soil as compared to control. Our results revealed that inoculation of beneficial microorganisms in combination with nanosilicon dioxide is an effective method for enhancing physicochemical and biochemical parameters of the soil which are responsible for increased plant growth and soil fertility by increasing enzyme activities of microbes. This approach presents an alternative to pesticides, fertilizers and GM crops to enhance crop productivity.
纳米化合物与促进植物生长的根际细菌一起应用，正受到越来越多关注以提高农业生产力。在本研究中，已尝试在盆栽试验中观察纳米二氧化硅（10毫克/升）以及两种促进植物生长的细菌（PC1-MK106029）和（PC4-MK106024）对玉米生长及其根际的影响。播种30天后，细菌联合体与纳米二氧化硅的联合处理使玉米的平均株高和叶片数量比对照有所增加。同样，在相同处理下，玉米叶片中总叶绿素、类胡萝卜素、糖、可溶性蛋白、酚类和黄酮类含量相对于对照的增幅分别为106%、307%、116%、57%、159%和132%。处理后的植株过氧化氢酶和过氧化物酶活性分别比对照显著提高了29.4%和73.9%。用植物生长促进根际细菌和纳米二氧化硅处理的土壤，其土壤健康的物理化学和生化参数也得到了改善。与对照相比，处理后的土壤中荧光素二乙酸酯、脱氢酶和碱性磷酸酶的活性增加了1.5至2倍。我们的结果表明，有益微生物与纳米二氧化硅联合接种是一种有效方法，可通过增加微生物的酶活性来提高土壤的物理化学和生化参数，而这些参数有助于植物生长和土壤肥力的提高。这种方法为提高作物生产力提供了一种替代农药、化肥和转基因作物的选择。
