Yu Liying, Tang Weiqi, He Weiyi, Ma Xiaoli, Vasseur Liette, Baxter Simon W, Yang Guang, Huang Shiguo, Song Fengqin, You Minsheng
1] Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China [2] Faculty of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China [3] Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou 350002, China.
1] Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China [2] Faculty of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Sci Rep. 2015 Mar 10;5:8952. doi: 10.1038/srep08952.
Cytochrome P450 monooxygenases are present in almost all organisms and can play vital roles in hormone regulation, metabolism of xenobiotics and in biosynthesis or inactivation of endogenous compounds. In the present study, a genome-wide approach was used to identify and analyze the P450 gene family of diamondback moth, Plutella xylostella, a destructive worldwide pest of cruciferous crops. We identified 85 putative cytochrome P450 genes from the P. xylostella genome, including 84 functional genes and 1 pseudogene. These genes were classified into 26 families and 52 subfamilies. A phylogenetic tree constructed with three additional insect species shows extensive gene expansions of P. xylostella P450 genes from clans 3 and 4. Gene expression of cytochrome P450s was quantified across multiple developmental stages (egg, larva, pupa and adult) and tissues (head and midgut) using P. xylostella strains susceptible or resistant to insecticides chlorpyrifos and fiprinol. Expression of the lepidopteran specific CYP367s predominantly occurred in head tissue suggesting a role in either olfaction or detoxification. CYP340s with abundant transposable elements and relatively high expression in the midgut probably contribute to the detoxification of insecticides or plant toxins in P. xylostella. This study will facilitate future functional studies of the P. xylostella P450s in detoxification.
细胞色素P450单加氧酶存在于几乎所有生物体中，在激素调节、外源化合物代谢以及内源性化合物的生物合成或失活过程中发挥着至关重要的作用。在本研究中，我们采用全基因组方法对小菜蛾（Plutella xylostella）的P450基因家族进行了鉴定和分析，小菜蛾是一种对十字花科作物具有全球毁灭性危害的害虫。我们从小菜蛾基因组中鉴定出85个假定的细胞色素P450基因，其中包括84个功能基因和1个假基因。这些基因被分为26个家族和52个亚家族。与另外三种昆虫构建的系统发育树显示，小菜蛾来自第3和第4族的P450基因有广泛的基因扩增。使用对杀虫剂毒死蜱和氟虫腈敏感或抗性的小菜蛾品系，对细胞色素P450在多个发育阶段（卵、幼虫、蛹和成虫）和组织（头部和中肠）中的基因表达进行了定量分析。鳞翅目特异性CYP367s主要在头部组织中表达，表明其在嗅觉或解毒中发挥作用。具有丰富转座元件且在中肠中表达相对较高的CYP340s可能有助于小菜蛾对杀虫剂或植物毒素的解毒。本研究将有助于未来对小菜蛾P450在解毒方面的功能研究。
