Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, PR China; State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan 611130, PR China.
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, PR China.
Plant Sci. 2020 Dec;301:110688. doi: 10.1016/j.plantsci.2020.110688. Epub 2020 Sep 28.
Stripe rust caused by Puccinia striiformis f. sp. tritici (Pst) is one of the most devastating foliar diseases in wheat. Host resistance is the most effective strategy for the management of the disease. To screen for accessions with stable resistance and identify effective stripe rust resistance loci, a genome-wide association study (GWAS) was conducted using a panel of 140 Chinese wheat landraces. The panel was evaluated for stripe rust response at the adult-plant stage at six field-year environments with mixed races and at the seedling stage with two separate predominant races of the pathogen, and genotyped with the genome-wide Diversity Arrays Technology markers. The panel displayed abundant phenotypic variation in stripe rust responses, with 9 landraces showing stable resistance to the mixture of Pst races at the adult-plant stage in the field and 10 landraces showing resistance to individual races at the seedling stage in the greenhouse. GWAS identified 12 quantitative trait loci (QTL) significantly (P ≤ 0.001) associated to stripe rust resistance using the field data of at least two environments and 18 QTL using the seedling data with two races. Among these QTL, 10 were presumably novel, including 4 for adult-plant resistance mapped to chromosomes 1B (QYrcl.sicau-1B.3), 4A (QYrcl.sicau-4A.3), 6A (QYrcl.sicau-6A.2) and 7B (QYrcl.sicau-7B.2) and 6 for all-stage resistance mapped to chromosomes 2D (QYrcl.sicau-2D.1), 3B (QYrcl.sicau-3B.3), 3D (QYrcl.sicau-3D), 4B (QYrcl.sicau-4B), 6A (QYrcl.sicau-6A.1) and 6D (QYrcl.sicau-6D). The landraces with stable resistance can be used for developing wheat cultivars with effective resistance to stripe rust.
条锈病由条形柄锈菌小麦专化型（Pst）引起，是小麦叶片最严重的病害之一。利用寄主抗性是防治该病最有效的策略。为了筛选具有稳定抗性的品系并鉴定有效的条锈病抗性基因座，本研究利用 140 份中国小麦地方品种进行了全基因组关联研究（GWAS）。该群体在 6 个田间年度环境中进行了成株期抗条锈病反应评估，采用混合菌系；在温室中采用 2 个主要菌系进行了苗期抗条锈病反应评估，并利用全基因组多样性阵列技术（Diversity Arrays Technology，DArT）标记进行了基因型分析。该群体在条锈病反应方面表现出丰富的表型变异，其中 9 个品系在成株期田间混合菌系下表现出稳定的抗性，10 个品系在温室苗期对单个菌系表现出抗性。利用至少 2 个环境的田间数据和 2 个菌系的苗期数据，GWAS 分别鉴定到与条锈病抗性显著相关（P≤0.001）的 12 个和 18 个数量性状位点（quantitative trait loci，QTL）。这些 QTL 中有 10 个可能是新的，包括 4 个成株期抗性 QTL，分别位于染色体 1B（QYrcl.sicau-1B.3）、4A（QYrcl.sicau-4A.3）、6A（QYrcl.sicau-6A.2）和 7B（QYrcl.sicau-7B.2）上，6 个全生育期抗性 QTL 位于染色体 2D（QYrcl.sicau-2D.1）、3B（QYrcl.sicau-3B.3）、3D（QYrcl.sicau-3D）、4B（QYrcl.sicau-4B）、6A（QYrcl.sicau-6A.1）和 6D（QYrcl.sicau-6D）上。这些具有稳定抗性的地方品种可用于培育具有有效抗性的小麦品种。
