Whelan W L, Markie D M, Simpkin K G, Poulter R M
J Bacteriol. 1985 Mar;161(3):1131-6. doi: 10.1128/jb.161.3.1131-1136.1985.
Total cellular DNA content, determined by a colorimetric method, was used as an index of ploidy in Candida albicans. Mononucleate hybrids were formed by fusion of spheroplasts derived from diploid parent strains. Five hybrids, of six studied, were taken to be tetraploid on the basis of estimated DNA content. One hybrid was taken to be hexaploid or near-hexaploid. Selection for increased resistance to 5-fluorocytosine in the hybrids, which were heterozygous for resistance, resulted in isolation of variants which were of lower ploidy than the hybrids from which they originated. Variants were obtained which corresponded (in measured DNA content) to aneuploid, triploid, and diploid states. These results may form the basis of a cyclic parasexual system (2n X 2n----4n----2n) for genetic analysis of this asexual species.
采用比色法测定的总细胞DNA含量，被用作白色念珠菌倍性的指标。单核杂种是由源自二倍体亲本菌株的原生质体融合形成的。在所研究的六个杂种中，根据估计的DNA含量，有五个被认为是四倍体。有一个杂种被认为是六倍体或接近六倍体。在对具有抗性杂合性的杂种中增加对5-氟胞嘧啶的抗性进行选择时，导致分离出比其起源的杂种倍性更低的变体。获得了与非整倍体、三倍体和二倍体状态相对应（在测量的DNA含量方面）的变体。这些结果可能构成用于对这个无性物种进行遗传分析的循环准性生殖系统（2n×2n→4n→2n）的基础。
