Li Yong, Jiao Yilai, Li Xiaokang, Guo Zheng
Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, People's Republic of China.
Shenyang National Laboratory for Materials Science, Institute of Metal Research, Chinese Academy of Sciences, Shenyang, Liaoning 110016, People's Republic of China.
Biochem Biophys Res Commun. 2015 May 1;460(2):151-6. doi: 10.1016/j.bbrc.2015.02.157. Epub 2015 Mar 7.
Osteointegration is crucial for success in orthopedic implantation. In recent decades, there have been numerous studies aiming to modify titanium alloys, which are the most widely used materials in orthopedics. Zeolites are solid aluminosilicates whose application in the biomedical field has recently been explored. To this end, MFI zeolites have been developed as titanium alloy coatings and tested in vitro. Nevertheless, the effect of the MFI coating of biomaterials in vivo has not yet been addressed. The aim of the present work is to evaluate the effects of MFI-coated Ti6Al4V implants in vitro and in vivo. After surface modification, the surface was investigated using field emission scanning electron microscopy (FE-SEM) and energy dispersive spectroscopy (EDS). No difference was observed regarding the proliferation of MC3T3-E1 cells on the Ti6Al4V (Ti) and MFI-coated Ti6Al4V (M-Ti) (p > 0.05). However, the attachment of MC3T3-E1 cells was found to be better in the M-Ti group. Additionally, ALP staining and activity assays and quantitative real-time RT-PCR indicated that MC3T3-E1 cells grown on the M-Ti displayed high levels of osteogenic differentiation markers. Moreover, Van-Gieson staining of histological sections demonstrated that the MFI coating on Ti6Al4V scaffolds significantly enhanced osteointegration and promoted bone regeneration after implantation in rabbit femoral condylar defects at 4 and 12 weeks. Therefore, this study provides a method for modifying Ti6Al4V to achieve improved osteointegration and osteogenesis.
骨整合对于骨科植入的成功至关重要。近几十年来，已有大量研究旨在对钛合金进行改性，钛合金是骨科中使用最广泛的材料。沸石是固体铝硅酸盐，其在生物医学领域的应用最近已得到探索。为此，已开发出MFI沸石作为钛合金涂层并进行了体外测试。然而，生物材料的MFI涂层在体内的作用尚未得到研究。本研究的目的是评估MFI涂层的Ti6Al4V植入物在体外和体内的效果。表面改性后，使用场发射扫描电子显微镜（FE-SEM）和能量色散光谱（EDS）对表面进行了研究。在Ti6Al4V（Ti）和MFI涂层的Ti6Al4V（M-Ti）上，MC3T3-E1细胞的增殖没有差异（p>0.05）。然而，发现MC3T3-E1细胞在M-Ti组中的附着更好。此外，碱性磷酸酶染色、活性测定和定量实时RT-PCR表明，在M-Ti上生长的MC3T3-E1细胞显示出高水平 的成骨分化标志物。此外，组织学切片的Van-Gieson染色表明，Ti6Al4V支架上的MFI涂层在植入兔股骨髁缺损4周和12周后，显著增强了骨整合并促进了骨再生。因此，本研究提供了一种改性Ti6Al4V以实现改善骨整合和成骨的方法。
