Department of Surgery and Integrated Clinic, Araçatuba Dental School, São Paulo State University Júlio de Mesquita Filho, São Paulo, Brazil.
Implant Dentistry Postgraduate Program, São Leopoldo Mandic School of Dentistry and Research Center, Campinas, Brazil.
Clin Oral Investig. 2019 Dec;23(12):4383-4397. doi: 10.1007/s00784-019-02872-6. Epub 2019 Apr 10.
The purpose of this study was to evaluate the biomechanical behavior of the interface formed between bone and implants with machined surfaces (MS) and those modified by AlO sandblasting and acid etching (SBAS).
Before surgery, topographic characterization was performed by SEM-EDX and by mean roughness measurements. Ten Albinus rabbits received randomly 20 Ti-6Al-4V implants on its right and left tibiae, with one implant placed in each tibia. After implant insertion, the implant stability quotient (ISQ) was measured by means of resonance frequency analysis (RFA). After 3 and 6 weeks, the ISQ was again measured, followed by torque removal measurements. Analysis of variance and Tukey tests were used to analyze the data. The surface of the implants removed was evaluated by SEM-EDX. Immunohistochemical analysis of osteopontin (OPN) and osteocalcin (OC) protein was performed in bone tissue.
The topographic characterization showed differences between the analyzed surfaces, and the mean roughness values of SBAS group were statistically higher than MS. Overall, higher statistically significant ISQ values were observed in the SBAS group compared to the MS group (p = 0.012). The intra-group comparison of ISQ values in the SBAS group showed statistically significant differences between 0 and 3 weeks (p = 0.032) and 0 and 6 weeks (p = 0.003). The torque removal measurements of group SBAS were statistically higher when compared with the torque removal measurements of group MS in the time intervals of 3 weeks (p = 0.002) and 6 weeks (p < 0.001). SEM-EDX of the implant surfaces removed in SBAS group showed greater bone tissue covering and mean values atomic in percentage of Ca, P, and O statistically superior (p < 0.05) than MS group. Immunohistochemical reactions showed intense OC immunolabeling at 6 weeks postoperative for SBAS group.
The topographical modifications made in group SBAS allowed a better mechanical interlocking between the implant and bone tissue.
本研究旨在评估经机械加工表面（MS）和 AlO 喷砂酸蚀（SBAS）处理的骨与植入物界面的生物力学行为。
手术前，通过 SEM-EDX 和平均粗糙度测量对形貌特征进行了表征。10 只 Albino 兔随机将 20 个 Ti-6Al-4V 植入物分别植入右侧和左侧胫骨，每侧胫骨植入一个。植入后，通过共振频率分析（RFA）测量植入物稳定性系数（ISQ）。3 周和 6 周后，再次测量 ISQ，并进行扭矩去除测量。采用方差分析和 Tukey 检验对数据进行分析。去除的植入物表面通过 SEM-EDX 进行评估。对骨组织中的骨桥蛋白（OPN）和骨钙素（OC）蛋白进行免疫组织化学分析。
形貌特征分析显示分析表面之间存在差异，SBAS 组的平均粗糙度值具有统计学意义的更高。总体而言，与 MS 组相比，SBAS 组的 ISQ 值更高且具有统计学意义（p = 0.012）。SBAS 组的 ISQ 值组内比较显示，0 至 3 周（p = 0.032）和 0 至 6 周（p = 0.003）之间存在统计学差异。在 3 周（p = 0.002）和 6 周（p < 0.001）的时间间隔内，SBAS 组的扭矩去除测量值明显高于 MS 组。在 SBAS 组中，从植入物表面去除的 SEM-EDX 显示出更大的骨组织覆盖，且 Ca、P 和 O 的原子百分平均值具有统计学意义的更高（p < 0.05）。免疫组织化学反应显示，术后 6 周 SBAS 组 OC 免疫标记强烈。
SBAS 组的形貌修饰允许植入物和骨组织之间更好的机械锁合。
