Duebel Jens, Marazova Katia, Sahel José-Alain
aInstitut de la Vision, Sorbonne Universités, UPMC Univ Paris, UMR_S 968 bINSERM, U_968 cCNRS, UMR_7210 dCentre Hospitalier National d'Ophtalmologie des Quinze-Vingts, DHU ViewMaintain, INSERM-DHOS CIC 1423 eFondation Ophtalmologique Rothschild, Paris, France fInstitute of Ophthalmology-University College London, London, UK.
Curr Opin Ophthalmol. 2015 May;26(3):226-32. doi: 10.1097/ICU.0000000000000140.
In this review, we will discuss the recent developments in optogenetics and their potential applications in ophthalmology to restore vision in retinal degenerative diseases.
In recent years, we have seen major advances in the field of optogenetics, providing us with novel opsins for potential applications in the retina. Microbial opsins with improved light sensitivity and red-shifted action spectra allow optogenetic stimulation at light levels well below the safety threshold in the human eye. In parallel, remarkable success in the development of highly efficient viral vectors for ocular gene therapy led to new strategies of using these novel optogenetic tools for vision restoration.
These recent findings show that novel optogenetic tools and viral vectors for ocular gene delivery are now available providing many opportunities to develop potential optogenetic strategies for vision restoration.
在本综述中，我们将讨论光遗传学的最新进展及其在眼科的潜在应用，以恢复视网膜退行性疾病中的视力。
近年来，我们见证了光遗传学领域的重大进展，为我们提供了可用于视网膜潜在应用的新型视蛋白。具有更高光敏感性和红移作用光谱的微生物视蛋白使得在远低于人眼安全阈值的光照水平下进行光遗传学刺激成为可能。与此同时，高效眼部基因治疗病毒载体开发方面取得的显著成功带来了使用这些新型光遗传学工具进行视力恢复的新策略。
这些最新发现表明，用于眼部基因递送的新型光遗传学工具和病毒载体现已具备，为开发潜在的视力恢复光遗传学策略提供了诸多机会。
