Mokso Rajmund, Schwyn Daniel A, Walker Simon M, Doube Michael, Wicklein Martina, Müller Tonya, Stampanoni Marco, Taylor Graham K, Krapp Holger G
Swiss Light Source, Paul Scherrer Institut, Villigen, Switzerland.
1] Department of Bioengineering, Imperial College London, UK [2] Department of Zoology, University of Oxford, UK.
Sci Rep. 2015 Mar 12;5:8727. doi: 10.1038/srep08727.
Visualizing fast micrometer scale internal movements of small animals is a key challenge for functional anatomy, physiology and biomechanics. We combine phase contrast tomographic microscopy (down to 3.3 μm voxel size) with retrospective, projection-based gating (in the order of hundreds of microseconds) to improve the spatiotemporal resolution by an order of magnitude over previous studies. We demonstrate our method by visualizing 20 three-dimensional snapshots through the 150 Hz oscillations of the blowfly flight motor.
可视化小动物快速的微米级内部运动是功能解剖学、生理学和生物力学面临的一项关键挑战。我们将相衬断层显微镜（体素大小低至3.3μm）与基于投影的回顾性门控技术（数百微秒量级）相结合，从而使时空分辨率比以往研究提高一个数量级。我们通过可视化家蝇飞行运动150Hz振荡过程中的20个三维快照来展示我们的方法。
