Amiri Negin, Abiri Ramin, Eyvazi Masoumeh, Zolfaghari Mohammad Reza, Alvandi Amirhooshang
Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran.
Department of Microbiology, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Arch Oral Biol. 2015 May;60(5):782-8. doi: 10.1016/j.archoralbio.2015.02.006. Epub 2015 Feb 21.
The commonest bacteria, causing infection across the world is Helicobacter pylori, which colonizes the human stomach. This bacteria has also been detected in some extra-gastric ecological niches such as the oral cavity and water. However, the results of H. pylori detection in extra-gastric ecological niche are controversial. The improvement of the sensitivity and the specificity of the detection methods appear to be some of the main bottleneck issues in providing compelling evidence. The aim of this study was to detect the presence of this organism in dental plaque samples using an analytically sensitive and specific Polymerase Chain Reaction (PCR) as well as a new nucleic acid detection method termed the Loop-mediated Isothermal Amplification (LAMP).
In a descriptive cross-sectional study 45 participants enrolled and dental plaque samples were collected from at least two teeth surfaces (one anterior and one posterior tooth) using a sterile periodontal curette. The DNA content was extracted from the samples and the presence of H. pylori was determined by PCR and LAMP reactions.
The frequency of detection of H. pylori in the dental plaque samples were 44% (20/45), 66.67% (30/45) and 77.78% (35/45) using PCR, LAMP and positivity for both tests, respectively.
The high frequency of H. pylori was detected in the dental plaque samples of the participants, which concurs with the high prevalence of this bacteria in the population. This is one of the highest reported rates around the world. The results reveal that dental plaque can be one of the main causes of re-infection and also be the cause of oral-oral transmission.
全球范围内引起感染最常见的细菌是幽门螺杆菌，它定植于人的胃部。在一些胃外生态位，如口腔和水中也检测到了这种细菌。然而，在胃外生态位检测幽门螺杆菌的结果存在争议。检测方法的灵敏度和特异性的提高似乎是提供确凿证据的一些主要瓶颈问题。本研究的目的是使用分析灵敏且特异的聚合酶链反应（PCR）以及一种称为环介导等温扩增（LAMP）的新型核酸检测方法，检测牙菌斑样本中该微生物的存在。
在一项描述性横断面研究中，招募了45名参与者，并使用无菌牙周刮匙从至少两个牙齿表面（一颗前牙和一颗后牙）采集牙菌斑样本。从样本中提取DNA含量，并通过PCR和LAMP反应确定幽门螺杆菌的存在。
使用PCR、LAMP以及两种检测均呈阳性时，牙菌斑样本中幽门螺杆菌的检测频率分别为44%（20/45）、66.67%（30/45）和77.78%（35/45）。
在参与者的牙菌斑样本中检测到幽门螺杆菌的频率很高，这与该细菌在人群中的高流行率一致。这是世界范围内报道的最高发生率之一。结果表明，牙菌斑可能是再次感染的主要原因之一，也是口口传播的原因。
