Chua Eng-Guan, Chong Ju-Yee, Lamichhane Binit, Webberley K Mary, Marshall Barry J, Wise Michael J, Tay Chin-Yen
The Marshall Centre for Infectious Diseases Research and Training, University of Western Australia, Crawley, Western Australia, Australia.
Shenzhen Dapeng New District Kuichong People Hospital, Shenzhen City, Guangdong Province, China.
PeerJ. 2019 Jan 28;7:e6336. doi: 10.7717/peerj.6336. eCollection 2019.
We investigated the effects of gastric infection on the daytime and overnight human oral microbiota.
Twenty four volunteers were recruited. Ten tested positive for infection by the Carbon-14 Urea Breath Test, and the rest were negative. Two oral swabs were collected: one immediately after waking up in the morning and before brushing teeth, and another in the evening before teeth-brushing. DNA extract acquired from each swab was subjected to Illumina sequencing of gene amplicons. The microbial abundance and composition were analysed in relation to infection status.
-positive individuals had significant changes in the alpha and beta diversities in the daytime samples in comparison to those who were negative. To identify which taxa could be significantly affected within the cohorts in the daytime, we employed the LEfSe method. When compared against UBT-negative samples, significantly higher abundances were detected in both and , while , , and were significantly decreased in the UBT-positive samples.
Our data demonstrated that infection affects the human daytime oral microbiota. The hitherto undocumented changes of several bacterial genera due to infection require more studies to examine their potential health effects on affected individuals.
我们研究了胃部感染对人类日间和夜间口腔微生物群的影响。
招募了24名志愿者。通过碳-14尿素呼气试验，10人检测出感染呈阳性，其余为阴性。采集两份口腔拭子：一份在早上醒来后、刷牙前立即采集，另一份在晚上刷牙前采集。从每份拭子中提取的DNA进行基因扩增子的Illumina测序。分析微生物丰度和组成与感染状态的关系。
与未感染的个体相比，感染阳性个体的日间样本在α和β多样性方面有显著变化。为了确定日间队列中哪些分类群可能受到显著影响，我们采用了线性判别分析效应大小（LEfSe）方法。与尿素呼气试验（UBT）阴性样本相比，在[具体菌属1]和[具体菌属2]中检测到显著更高的丰度，而在UBT阳性样本中，[具体菌属3]、[具体菌属4]、[具体菌属5]和[具体菌属6]显著减少。
我们的数据表明，胃部感染会影响人类日间口腔微生物群。由于胃部感染导致的几个细菌属的迄今未记录的变化需要更多研究来检查它们对受影响个体的潜在健康影响。
