Aytac Zeynep, Sen Huseyin Sener, Durgun Engin, Uyar Tamer
Institute of Materials Science & Nanotechnology, Bilkent University, Ankara 06800, Turkey; UNAM-National Nanotechnology Research Center, Bilkent University, Ankara 06800, Turkey.
UNAM-National Nanotechnology Research Center, Bilkent University, Ankara 06800, Turkey.
Colloids Surf B Biointerfaces. 2015 Apr 1;128:331-338. doi: 10.1016/j.colsurfb.2015.02.019. Epub 2015 Feb 17.
Herein, hydroxypropyl-beta-cyclodextrin (HPβCD) inclusion complex (IC) of a hydrophobic drug, sulfisoxazole (SFS) was incorporated in hydroxypropyl cellulose (HPC) nanofibers (HPC/SFS/HPβCD-IC-NF) via electrospinning. SFS/HPβCD-IC was characterized by DSC to investigate the formation of inclusion complex and the stoichiometry of the complex was determined by Job's plot. Modeling studies were also performed on SFS/HPβCD-IC using ab initio technique. SEM images depicted the defect free uniform fibers and confirmed the incorporation of SFS/HPβCD-IC in nanofibers did not alter the fiber morphology. XRD analyses showed amorphous distribution of SFS/HPβCD-IC in the fiber mat. Release studies were performed in phosphate buffered saline (PBS). The results suggest higher amount of SFS released from HPC/SFS/HPβCD-IC-NF when compared to free SFS containing HPC nanofibers (HPC/SFS-NF). This was attributed to the increased solubility of SFS by inclusion complexation. Sandwich configurations were prepared by placing HPC/SFS/HPβCD-IC-NF between electrospun PCL nanofibrous mat (PCL-HPC/SFS/HPβCD-IC-NF). Consequently, PCL-HPC/SFS/HPβCD-IC-NF exhibited slower release of SFS as compared with HPC/SFS/HPβCD-IC-NF. This study may provide more efficient future strategies for developing delivery systems of hydrophobic drugs.
在此，通过静电纺丝将疏水性药物磺胺异噁唑（SFS）的羟丙基-β-环糊精（HPβCD）包合物（IC）掺入羟丙基纤维素（HPC）纳米纤维（HPC/SFS/HPβCD-IC-NF）中。通过差示扫描量热法（DSC）对SFS/HPβCD-IC进行表征，以研究包合物的形成，并通过Job曲线确定该配合物的化学计量比。还使用从头算技术对SFS/HPβCD-IC进行了建模研究。扫描电子显微镜（SEM）图像描绘了无缺陷的均匀纤维，并证实SFS/HPβCD-IC掺入纳米纤维中并未改变纤维形态。X射线衍射（XRD）分析表明SFS/HPβCD-IC在纤维毡中呈无定形分布。在磷酸盐缓冲盐水（PBS）中进行了释放研究。结果表明，与含游离SFS的HPC纳米纤维（HPC/SFS-NF）相比，从HPC/SFS/HPβCD-IC-NF中释放出的SFS量更高。这归因于包合络合作用使SFS的溶解度增加。通过将HPC/SFS/HPβCD-IC-NF置于静电纺丝的聚己内酯（PCL）纳米纤维毡之间制备三明治结构（PCL-HPC/SFS/HPβCD-IC-NF）。因此，与HPC/SFS/HPβCD-IC-NF相比，PCL-HPC/SFS/HPβCD-IC-NF表现出较慢的SFS释放。该研究可能为开发疏水性药物递送系统提供更有效的未来策略。
