Peppelman M, van den Eijnde W A J, Jaspers E J, Gerritsen M-J P, van Erp P E J
Department of Dermatology, Radboud University Medical Center, Nijmegen, The Netherlands.
Skin Res Technol. 2015 Nov;21(4):474-84. doi: 10.1111/srt.12217. Epub 2015 Mar 12.
Evaluation of (immuno)histological and cell biological changes in damaged skin requires often an invasive skin biopsy, making in vivo models inappropriate to study skin damage. Reflectance confocal microscopy (RCM) might overcome this limitation. Therefore, we evaluated the use of a tape-stripping model in combination with RCM to provide morphological data on skin damage and recovery.
In 25 volunteers, a tape-stripping stimulus was applied. The skin was imaged with RCM during 1 week and 3 mm punch biopsies were obtained.
Strong correlations between epidermal thickness determined by RCM and conventional histological measurements were found. RCM thickness measurements correlated well with epidermal proliferation. The 10× or 15× repeated tape-stripping resulted in skin damage similar to acute stripping. Mild repeated tape-stripping showed no skin damage.
Overall, we demonstrated that non-invasive RCM in combination with tape-stripping could be used as model to obtain morphological and cell biological data on skin-material interactions.
评估受损皮肤的（免疫）组织学和细胞生物学变化通常需要进行侵入性皮肤活检，这使得体内模型不适用于研究皮肤损伤。反射式共聚焦显微镜（RCM）可能会克服这一局限性。因此，我们评估了胶带剥离模型与RCM结合使用，以提供有关皮肤损伤和恢复的形态学数据。
对25名志愿者施加胶带剥离刺激。在1周内用RCM对皮肤进行成像，并获取3毫米的打孔活检样本。
发现RCM测定的表皮厚度与传统组织学测量之间存在强相关性。RCM厚度测量与表皮增殖密切相关。10次或15次重复胶带剥离导致的皮肤损伤类似于急性剥离。轻度重复胶带剥离未显示皮肤损伤。
总体而言，我们证明了非侵入性RCM与胶带剥离相结合可作为获取皮肤-材料相互作用的形态学和细胞生物学数据的模型。
