Wang Zhen, Yang Yong, Yuan Li-jun, Liu Jie, Duan Yun-you, Cao Tie-sheng
Department of Ultrasound Diagnostics, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.
PLoS One. 2015 Mar 18;10(3):e0120482. doi: 10.1371/journal.pone.0120482. eCollection 2015.
To evaluate the validity and reproducibility of a noninvasive dual pulse wave Doppler (DPWD) method, which involves simultaneous recording of flow velocity of two independent sample volumes with a measurable distance, for measuring the local arterial pulse wave velocity (PWV) through in vitro and in vivo studies.
The DPWD mode of Hitachi HI Vision Preirus ultrasound system with a 5-13MHz transducer was used. An in vitro model was designed to compare the PWV of a homogeneous rubber tubing with the local PWV of its middle part measured by DPWD method. In the in vivo study, local PWV of 45 hypertensive patients (25 male, 49.8±3.1 years) and 45 matched healthy subjects (25 male, 49.3±3.0 years) were investigated at the left common carotid artery (LCCA) by DPWD method.
In the in vitro study, the local PWV measured by DPWP method and the PWV of the homogeneous rubber tubing did not show statistical difference (5.16 ± 0.28 m/s vs 5.03 ± 0.15 m/s, p = 0.075). The coefficient of variation (CV) of the intra- and inter- measurements for local PWV were 3.46% and 4.96%, for the PWV of the homogeneous rubber tubing were 0.99% and 1.98%. In the in vivo study, a significantly higher local PWV of LCCA was found in the hypertensive patients as compared to that in healthy subjects (6.29±1.04 m/s vs. 5.31±0.72 m/s, P = 0.019). The CV of the intra- and inter- measurements in hypertensive patients were 2.22% and 3.94%, in healthy subjects were 2.07% and 4.14%.
This study demonstrated the feasibility of the noninvasive DPWD method to determine the local PWV, which was accurate and reproducible not only in vitro but also in vivo studies. This noninvasive echocardiographic method may be illuminating to clinical use.
通过体外和体内研究，评估一种非侵入性双脉冲波多普勒（DPWD）方法的有效性和可重复性，该方法涉及同时记录两个具有可测量距离的独立样本容积的流速，以测量局部动脉脉搏波速度（PWV）。
使用配备5-13MHz换能器的日立HI Vision Preirus超声系统的DPWD模式。设计了一个体外模型，将均匀橡胶管的PWV与其通过DPWD方法测量的中间部分的局部PWV进行比较。在体内研究中，采用DPWD方法对45例高血压患者（男性25例，年龄49.8±3.1岁）和45例匹配的健康受试者（男性25例，年龄49.3±3.0岁）的左颈总动脉（LCCA）局部PWV进行研究。
在体外研究中，DPWP方法测量的局部PWV与均匀橡胶管的PWV无统计学差异（5.16±0.28m/s对5.03±0.15m/s，p = 0.075）。局部PWV测量的组内和组间变异系数（CV）分别为3.46%和4.96%，均匀橡胶管PWV的组内和组间变异系数分别为0.99%和1.98%。在体内研究中，高血压患者LCCA的局部PWV显著高于健康受试者（6.29±1.04m/s对5.31±0.72m/s，P = 0.019）。高血压患者测量的组内和组间CV分别为2.22%和3.94%，健康受试者分别为并2.07%和4.14%。
本研究证明了非侵入性DPWD方法测定局部PWV的可行性，该方法不仅在体外研究中准确且可重复，在体内研究中也是如此。这种非侵入性超声心动图方法可能对临床应用具有启示意义。
