Department of Pharmacy and Pharmacology, University of Bath, Bath, BA2 7AY, UK.
Drug Deliv Transl Res. 2013 Dec;3(6):542-50. doi: 10.1007/s13346-013-0179-2.
We report the controlled release of the antibiotic tetracycline (Tet) from triple-layered (3L) electrospun matrices consisting of zein or a zein/PCL blend, where the drug was loaded into the central layer with the two outer layers acting as diffusion barriers. These fibrous matrices successfully encapsulated Tet and efficiently inhibited the growth of a clinical isolate, the methicillin-resistant Staphylococcus aureus strain MRSA252, as demonstrated in a modified Kirby-Bauer disc assay over 5 days. Whilst untreated zein fibres are unstable in an aqueous environment, rapidly shrinking due to plasticisation and film formation, blending zein with PCL stabilised the electrospun matrices and prevented them from shrinking. These 3L formulations display sustained antibiotic release and provide a proof of concept for zein-based polymeric matrices as wound dressings to treat or prevent bacterial infection. This is the first demonstration of the controlled release of a clinically used antibiotic from electrospun zein-based matrices.
我们报告了由三层（3L）电纺基质控制释放抗生素四环素（Tet）的情况，该基质由玉米醇溶蛋白或玉米醇溶蛋白/PCL 混合物组成，药物被加载到中央层，而两个外层作为扩散屏障。这些纤维基质成功地封装了 Tet，并在改良的 Kirby-Bauer 圆盘测定中在 5 天内有效抑制了临床分离株耐甲氧西林金黄色葡萄球菌菌株 MRSA252 的生长。虽然未经处理的玉米醇溶蛋白纤维在水环境中不稳定，由于塑化和薄膜形成而迅速收缩，但将玉米醇溶蛋白与 PCL 混合稳定了电纺基质并防止它们收缩。这些 3L 配方显示出持续的抗生素释放，并为基于玉米醇溶蛋白的聚合物基质作为治疗或预防细菌感染的伤口敷料提供了概念验证。这是首次从基于电纺玉米醇溶蛋白的基质中控制释放临床使用的抗生素的证明。
