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静电纺丝玉米醇溶蛋白/聚己内酯纤维基质以可控方式释放四环素，可杀死生物膜中的金黄色葡萄球菌以及猪皮上的离体金黄色葡萄球菌，并且与人皮肤细胞相容。

Electrospun Zein/PCL Fibrous Matrices Release Tetracycline in a Controlled Manner, Killing Staphylococcus aureus Both in Biofilms and Ex Vivo on Pig Skin, and are Compatible with Human Skin Cells.

作者信息

Alhusein Nour, Blagbrough Ian S, Beeton Michael L, Bolhuis Albert, De Bank Paul A

机构信息

Department of Pharmacy and Pharmacology, University of Bath, Bath, BA2 7AY, UK.

Department of Biomedical Sciences, Cardiff Metropolitan University, Western Avenue, Cardiff, CF5 2YB, UK.

出版信息

Pharm Res. 2016 Jan;33(1):237-46. doi: 10.1007/s11095-015-1782-3. Epub 2015 Sep 3.

DOI:10.1007/s11095-015-1782-3
PMID:26337770
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4689739/
Abstract

PURPOSE

To investigate the destruction of clinically-relevant bacteria within biofilms via the sustained release of the antibiotic tetracycline from zein-based electrospun polymeric fibrous matrices and to demonstrate the compatibility of such wound dressing matrices with human skin cells.

METHODS

Zein/PCL triple layered fibrous dressings with entrapped tetracycline were electrospun. The successful entrapment of tetracycline in these dressings was validated. The successful release of bioactive tetracycline, the destruction of preformed biofilms, and the viability of fibroblast (FEK4) cells were investigated.

RESULTS

The sustained release of tetracycline from these matrices led to the efficient destruction of preformed biofilms from Staphylococcus aureus MRSA252 in vitro, and of MRSA252 and ATCC 25923 bacteria in an ex vivo pig skin model using 1 × 1 cm square matrices containing tetracycline (30 μg). Human FEK4 cells grew normally in the presence of these matrices.

CONCLUSIONS

The ability of the zein-based matrices to destroy bacteria within increasingly complex in vitro biofilm models was clearly established. An ex vivo pig skin assay showed that these matrices, with entrapped tetracycline, efficiently kill bacteria and this, combined with their compatibility with a human skin cell line suggest these matrices are well suited for applications in wound healing and infection control.

摘要

目的

研究通过基于玉米醇溶蛋白的电纺聚合物纤维基质持续释放抗生素四环素，对生物膜内临床相关细菌的破坏作用，并证明此类伤口敷料基质与人类皮肤细胞的相容性。

方法

静电纺丝制备含有四环素的玉米醇溶蛋白/聚己内酯三层纤维敷料。验证了四环素在这些敷料中的成功包封。研究了生物活性四环素的成功释放、预先形成的生物膜的破坏以及成纤维细胞（FEK4）的活力。

结果

这些基质中四环素的持续释放导致体外金黄色葡萄球菌MRSA252以及使用含四环素（30μg）的1×1平方厘米方形基质的离体猪皮肤模型中MRSA252和ATCC 25923细菌预先形成的生物膜被有效破坏。人类FEK4细胞在这些基质存在的情况下正常生长。

结论

明确证实了基于玉米醇溶蛋白的基质在日益复杂的体外生物膜模型中破坏细菌 的能力。离体猪皮肤试验表明，这些含有四环素的基质能有效杀灭细菌，再加上它们与人类皮肤细胞系的相容性，表明这些基质非常适合用于伤口愈合和感染控制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b381/4689739/0b6dcfa1e9ed/11095_2015_1782_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b381/4689739/d265639b4683/11095_2015_1782_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b381/4689739/7c7f011c9d43/11095_2015_1782_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b381/4689739/b92889af2fe9/11095_2015_1782_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b381/4689739/00814c5c89ec/11095_2015_1782_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b381/4689739/a798cd9f06a7/11095_2015_1782_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b381/4689739/9211e89c530d/11095_2015_1782_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b381/4689739/0b6dcfa1e9ed/11095_2015_1782_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b381/4689739/d265639b4683/11095_2015_1782_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b381/4689739/7c7f011c9d43/11095_2015_1782_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b381/4689739/b92889af2fe9/11095_2015_1782_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b381/4689739/00814c5c89ec/11095_2015_1782_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b381/4689739/a798cd9f06a7/11095_2015_1782_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b381/4689739/9211e89c530d/11095_2015_1782_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b381/4689739/0b6dcfa1e9ed/11095_2015_1782_Fig7_HTML.jpg

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