Baker M E
FEBS Lett. 1985 Mar 11;182(1):47-52. doi: 10.1016/0014-5793(85)81151-0.
Using a computer program designed to detect evolutionary relationships between proteins, I find that residues 72-110 of the mature sequence of human tissue-type plasminogen activator (t-PA) and 39 residues at the carboxy terminus of human albumin have a comparison score that is 8.8 standard deviation units higher than that obtained with a comparison of randomized sequences of these proteins. The probability (p) of getting this score by chance is approximately 10(-18), indicating that part of t-PA and albumin are derived from a common ancestor. I also find that alpha-fetoprotein, a relative of albumin is related to t-PA. Part of this region on t-PA has been previously shown to be related to epidermal growth factor. t-PA, albumin, alpha-fetoprotein, and epidermal growth factor have diverse biological activities. The finding that these proteins are related suggests some new approaches for studying their functions.
通过使用一个旨在检测蛋白质之间进化关系的计算机程序，我发现人类组织型纤溶酶原激活剂（t-PA）成熟序列的72-110位残基与人白蛋白羧基末端的39个残基的比对分数，比这些蛋白质随机序列比对所得的分数高出8.8个标准差单位。偶然获得此分数的概率（p）约为10^(-18)，这表明t-PA和白蛋白的一部分源自共同祖先。我还发现，作为白蛋白亲属的甲胎蛋白与t-PA有关。此前已表明t-PA上该区域的一部分与表皮生长因子有关。t-PA、白蛋白、甲胎蛋白和表皮生长因子具有多种生物学活性。这些蛋白质相关的发现为研究它们的功能提供了一些新方法。
