Huang Boyang, Feng Mingbao, Li Dinglong, Yang Yan
School of Environmental & Safety Engineering, Changzhou University, Jiangsu, Changzhou 213164, PR China.
State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Jiangsu, Nanjing 210046, PR China.
Ecotoxicol Environ Saf. 2015 Jun;116:107-12. doi: 10.1016/j.ecoenv.2015.03.009. Epub 2015 Mar 17.
Di-n-butyl phthalate (DBP) and di-2-ethylhexyl phthalate (DEHP) are two kinds of widely-used phthalates, whereas Cu (II) is a common valence state of copper. They have been ubiquitously detected in the aquatic environment, but information on their joint toxicity on aquatic organisms is scarce. In this study, we evaluated the combined effects of copper and these two phthalates to the goldfish (Carassius auratus) by detecting the antioxidant responses in liver after exposure for 7 and 21 days. The exposure concentrations were in a range relevant to their levels in the natural aquatic environment. The results indicated that DBP, DEHP and Cu (II) can affect the antioxidant status in fish liver, evidenced by the significant alterations of antioxidant defenses (superoxide dismutase, catalase, glutathione) and malondialdehyde. Antagonistic effects were found in the joint toxicity of Cu (II) and DBP or DEHP using the integrated biomarker response (IBR) index. These findings have important implications in the risk assessments of phthalates mixed with some heavy metals in the aquatic environment.
邻苯二甲酸二丁酯（DBP）和邻苯二甲酸二（2-乙基己基）酯（DEHP）是两种广泛使用的邻苯二甲酸盐，而铜（II）是铜的一种常见价态。它们在水生环境中普遍被检测到，但关于它们对水生生物的联合毒性的信息却很少。在本研究中，我们通过检测暴露7天和21天后肝脏中的抗氧化反应，评估了铜与这两种邻苯二甲酸盐对金鱼（Carassius auratus）的联合影响。暴露浓度处于与自然水生环境中它们的水平相关的范围内。结果表明，DBP、DEHP和铜（II）会影响鱼肝中的抗氧化状态，抗氧化防御（超氧化物歧化酶、过氧化氢酶、谷胱甘肽）和丙二醛的显著变化证明了这一点。使用综合生物标志物反应（IBR）指数发现，铜（II）与DBP或DEHP的联合毒性具有拮抗作用。这些发现对水生环境中邻苯二甲酸盐与某些重金属混合的风险评估具有重要意义。
