Aravinthan Adithan, Govarthanan Muthusamy, Selvam Kandasamy, Praburaman Loganathan, Selvankumar Thangasamy, Balamurugan Rangachari, Kamala-Kannan Seralathan, Kim Jong-Hoon
College of Veterinary Medicine, Biosafety Research Institute, Chonbuk National University, Jeonju, South Korea.
Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South Korea ; PG and Research Department of Biotechnology, Mahendra Arts and Science College, Kalippatti, Namakkal, Tamil Nadu, India.
Int J Nanomedicine. 2015 Mar 11;10:1977-83. doi: 10.2147/IJN.S79106. eCollection 2015.
A rapid, green phytosynthesis of silver nanoparticles (AgNPs) using the aqueous extract of Helianthus tuberosus (sunroot tuber) was reported in this study. The morphology of the AgNPs was determined by transmission electron microscopy (TEM). Scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS) and X-ray powder diffraction (XRD) analysis confirmed the presence of AgNPs. Fourier transform infrared spectroscopy (FTIR) analysis revealed that biomolecules in the tuber extract were involved in the reduction and capping of AgNPs. The energy-dispersive spectroscopy (EDS) analysis of the AgNPs, using an energy range of 2-4 keV, confirmed the presence of elemental silver without any contamination. Further, the synthesized AgNPs were evaluated against phytopathogens such as Ralstonia solanacearum and Xanthomonas axonopodis. The AgNPs (1-4 mM) extensively reduced the growth rate of the phytopathogens. In addition, the cytotoxic effect of the synthesized AgNPs was analyzed using rat splenocytes. The cell viability was decreased according to the increasing concentration of AgNPs and 67% of cell death was observed at 100 μg/mL.
本研究报道了利用菊芋（洋姜块茎）水提取物快速、绿色地合成银纳米颗粒（AgNPs）。通过透射电子显微镜（TEM）确定了AgNPs的形态。扫描电子显微镜-能量色散光谱（SEM-EDS）和X射线粉末衍射（XRD）分析证实了AgNPs的存在。傅里叶变换红外光谱（FTIR）分析表明，块茎提取物中的生物分子参与了AgNPs的还原和包覆。使用2-4 keV能量范围对AgNPs进行的能量色散光谱（EDS）分析证实了元素银的存在且无任何污染。此外，对合成的AgNPs针对诸如青枯雷尔氏菌和野油菜黄单胞菌等植物病原体进行了评估。AgNPs（1-4 mM）显著降低了植物病原体的生长速率。另外，使用大鼠脾细胞分析了合成的AgNPs的细胞毒性作用。细胞活力随着AgNPs浓度的增加而降低，在100μg/mL时观察到67%的细胞死亡。
