Centre for Nanobiotechnology, VIT University, Vellore, India.
Environ Sci Pollut Res Int. 2015 Jul;22(14):11057-66. doi: 10.1007/s11356-015-4355-4. Epub 2015 Mar 21.
The commercial usage of Al2O3 nanoparticles (Al2O3 NPs) has gone up significantly in the recent times, enhancing the risk of environmental contamination with these agents and their consequent adverse effects on living systems. The current study has been designed to evaluate the cytogenetic potential of Al2O3 NPs in Allium cepa (root tip cells) at a range of exposure concentrations (0.01, 0.1, 1, 10, and 100 μg/mL), their uptake/internalization profile, and the oxidative stress generated. We noted a dose-dependent decrease in the mitotic index (42 to 28 %) and an increase in the number of chromosomal aberrations. Various chromosomal aberrations, e.g. sticky, multipolar and laggard chromosomes, chromosomal breaks, and the formation of binucleate cells, were observed by optical, fluorescence, and confocal laser scanning microscopy. FT-IR analysis demonstrated the surface chemical interaction between the nanoparticles and root tip cells. The biouptake of Al2O3 in particulate form led to reactive oxygen species generation, which in turn probably contributed to the induction of chromosomal aberrations.
近年来，Al2O3 纳米粒子（Al2O3 NPs）的商业用途显著增加，增加了这些制剂对环境造成污染的风险，以及对生物系统产生的不良影响。本研究旨在评估不同暴露浓度（0.01、0.1、1、10 和 100μg/mL）下 Al2O3 NPs 在洋葱根尖细胞中的细胞遗传学潜力，及其摄取/内化情况和产生的氧化应激。我们注意到有丝分裂指数（从 42%降至 28%）呈剂量依赖性下降，染色体畸变数量增加。通过光学显微镜、荧光显微镜和共聚焦激光扫描显微镜观察到各种染色体畸变，如粘性、多极和滞后染色体、染色体断裂以及双核细胞的形成。FT-IR 分析表明，纳米颗粒与根尖细胞之间存在表面化学相互作用。以颗粒形式摄入的 Al2O3 导致活性氧物质的产生，这可能促成了染色体畸变的诱导。
