Dambach Michael, Sandoval Melissa, Updegrove Taylor B, Anantharaman Vivek, Aravind L, Waters Lauren S, Storz Gisela
Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892-5430, USA.
National Center for Biotechnology Information, National Library of Medicine, Bethesda, MD 20894, USA.
Mol Cell. 2015 Mar 19;57(6):1099-1109. doi: 10.1016/j.molcel.2015.01.035.
The highly structured, cis-encoded RNA elements known as riboswitches modify gene expression upon binding a wide range of molecules. The yybP-ykoY motif was one of the most broadly distributed and numerous bacterial riboswitches for which the cognate ligand was unknown. Using a combination of in vivo reporter and in vitro expression assays, equilibrium dialysis, and northern analysis, we show that the yybP-ykoY motif responds directly to manganese ions in both Escherichia coli and Bacillus subtilis. The identification of the yybP-ykoY motif as a manganese ion sensor suggests that the genes that are preceded by this motif and encode a diverse set of poorly characterized membrane proteins have roles in metal homeostasis.
被称为核糖开关的高度结构化、顺式编码的RNA元件在结合多种分子后会改变基因表达。yybP-ykoY基序是分布最广泛且数量众多的细菌核糖开关之一，其同源配体尚不清楚。通过体内报告基因和体外表达分析、平衡透析以及Northern分析相结合的方法，我们表明yybP-ykoY基序在大肠杆菌和枯草芽孢杆菌中均直接响应锰离子。将yybP-ykoY基序鉴定为锰离子传感器表明，位于该基序之前并编码一组功能多样但特征不明的膜蛋白的基因在金属稳态中发挥作用。
