Department of Chemical Engineering and Food Technology, Faculty of Sciences, International Agri-Food Campus of Excellence (CeiA3), University of Cádiz, Polígono Río San Pedro s/n, Puerto Real, Spain.
Department of Biology, Sao Paulo State University-Unesp, São José do Rio Preto, São Paulo, Brazil.
Bioresour Technol. 2015 Jun;185:316-23. doi: 10.1016/j.biortech.2015.02.112. Epub 2015 Mar 6.
A pretreatment with microwave irradiation was applied to enhance enzyme hydrolysis of corn straw and rice husk immersed in water, aqueous glycerol or alkaline glycerol. Native and pretreated solids underwent enzyme hydrolysis using the extract obtained from the fermentation of Myceliophthora heterothallica, comparing its efficiency with that of the commercial cellulose cocktail Celluclast®. The highest saccharification yields, for both corn straw and rice husk, were attained when biomass was pretreated in alkaline glycerol, method that has not been previously reported in literature. Moreover, FTIR, TG and SEM analysis revealed a more significant modification in the structure of corn straw subjected to this pretreatment. Highest global yields were attained with the crude enzyme extract, which might be the result of its content in a great variety of hydrolytic enzymes, as revealed zymogram analysis. Moreover, its hydrolysis efficiency can be improved by its supplementation with commercial β-glucosidase.
预处理时采用微波辐射来增强浸入水中、含水甘油或碱性甘油中的玉米秸秆和稻壳的酶水解。使用从嗜热真菌发酵获得的提取液对天然和预处理的固体进行酶水解，比较其效率与商业纤维素鸡尾酒 Celluclast®的效率。对于玉米秸秆和稻壳，当生物质在碱性甘油中预处理时，可获得最高的糖化产率，而该方法在文献中尚未报道过。此外，FTIR、TG 和 SEM 分析表明，经过这种预处理后，玉米秸秆的结构发生了更显著的变化。最高的总产率是用粗酶提取物获得的，这可能是由于它含有大量的水解酶，如酶谱分析所示。此外，通过补充商业β-葡萄糖苷酶可以提高其水解效率。
