茶科微卫星标记的开发
Development of microsatellite markers for (Theaceae).
作者信息
Shi Xianggang, Wu Haidan, Xie Hongxian, Zheng Yi, Huang Yelin, Yu Shixiao
机构信息
State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, Guangdong, People's Republic of China.
出版信息
Appl Plant Sci. 2017 Jul 21;5(7). doi: 10.3732/apps.1700037. eCollection 2017 Jul.
PREMISE OF THE STUDY
Sixteen microsatellite markers were developed to study the fine-scale spatial genetic structure of , a dioecious tree species of Theaceae endemic to southern China.
METHODS AND RESULTS
A total of 30 primer pairs were initially designed and tested on the basis of the transcriptome data of , of which 16 were successfully amplified and showed clear polymorphism. For these microsatellites, one to 17 alleles per locus were identified. The observed and expected heterozygosities ranged from 0 to 1.000 and 0 to 0.903, respectively.
CONCLUSIONS
The microsatellite markers described here can be used to study genetic diversity and spatial genetic structure of . Furthermore, all loci were successfully cross-amplified in a related species, .
研究前提
开发了16个微卫星标记，用于研究中国南方特有的山茶科雌雄异株树种的精细空间遗传结构。
方法与结果
基于该树种的转录组数据，初步设计并测试了30对引物，其中16对成功扩增并表现出明显的多态性。对于这些微卫星，每个位点鉴定出1至17个等位基因。观察到的杂合度和期望杂合度分别在0至1.000和0至0.903之间。
结论
本文所述的微卫星标记可用于研究该树种的遗传多样性和空间遗传结构。此外，所有位点在一个相关物种中都成功进行了交叉扩增。
Zotero 插件