Kšonžeková Petra, Mariychuk Ruslan, Eliašová Adriana, Mudroňová Dagmar, Csank Tomáš, Király Ján, Marcinčáková Dana, Pistl Juraj, Tkáčiková L'udmila
Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Košice, Slovakia.
Department of Ecology, Faculty of Humanities and Natural Sciences, University of Prešov, Prešov, Slovakia.
J Sci Food Agric. 2016 Mar 15;96(4):1093-100. doi: 10.1002/jsfa.7181. Epub 2015 Apr 24.
Anthocyanins, compounds that represent the major group of flavonoids in berries, are one of the most powerful natural antioxidants. The aim of this study was to evaluate biological activities and comparison of anthocyanin-rich extracts prepared from chokeberry (Aronia melanocarpa), elderberry (Sambucus nigra), bilberry (Vaccinium myrtillus) and blueberry (V. corymbosum) on the porcine intestinal epithelial IPEC-1 cell line.
The IC50 values calculated in the antioxidant cell-based dichlorofluorescein assay (DCF assay) were 1.129 mg L(-1) for chokeberry, 1.081 mg L(-1) for elderberry, 2.561 mg L(-1) for bilberry and 2.965 mg L(-1) for blueberry, respectively. We found a significant negative correlation (P < 0.001) between cyanidin glycosides content and IC50 values. Moreover, extracts rich in cyanidin glycosides stimulated proliferation of IPEC-1 cells and did not have cytotoxic effect on cells at an equivalent in vivo concentration.
We found that the chokeberry and elderberry extracts rich in cyanidin glycosides possess better antioxidant and anticytotoxic activities in comparison to blueberry or bilberry extracts with complex anthocyanin profiles.
花青素是浆果中主要的黄酮类化合物，是最强大的天然抗氧化剂之一。本研究旨在评估从黑果腺肋花楸（Aronia melanocarpa）、接骨木果（Sambucus nigra）、欧洲越橘（Vaccinium myrtillus）和蓝莓（V. corymbosum）制备的富含花青素的提取物对猪肠道上皮IPEC-1细胞系的生物活性并进行比较。
在基于细胞的抗氧化二氯荧光素测定法（DCF测定法）中计算得到的IC50值，黑果腺肋花楸为1.129 mg L(-1)，接骨木果为1.081 mg L(-1)，欧洲越橘为2.561 mg L(-1)，蓝莓为2.965 mg L(-1)。我们发现矢车菊素糖苷含量与IC50值之间存在显著的负相关（P < 0.001）。此外，富含矢车菊素糖苷的提取物刺激了IPEC-1细胞的增殖，并且在等效体内浓度下对细胞没有细胞毒性作用。
我们发现，与具有复杂花青素谱的蓝莓或欧洲越橘提取物相比，富含矢车菊素糖苷的黑果腺肋花楸和接骨木果提取物具有更好的抗氧化和抗细胞毒性活性。
