Department of Bioenergetics, Food Analysis and Microbiology, Institute of Food Technology and Nutrition, University of Rzeszów, Zelwerowicza 4, 35-601 Rzeszów, Poland.
Department of Chemistry and Food Toxicology, Institute of Food Technology and Nutrition, University of Rzeszów, Ćwiklińskiej 1a, 35-601 Rzeszów, Poland.
Molecules. 2020 Nov 25;25(23):5517. doi: 10.3390/molecules25235517.
Plants from Asteraceae family are widely used for their therapeutic effects in the treatment of gastrointestinal diseases, but the consequences of excessive intake still need to be studied. The aims of this study were the evaluation of cytotoxicity, measurement of antioxidant properties and determination of polyphenolic profile of L. (tansy), L. (yarrow) and Ait. (goldenrod) ethanolic extracts. The cytotoxicity of extracts was monitored by xCELLigence system in real time by using porcine intestinal epithelial cell line (IPEC-1) and by measurement of changes in metabolic activity ((3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS) assay). The antioxidant properties were measured by spectrophotometric methods and polyphenolic profiles were determined by HPLC-DAD for 50% ethanol extracts (10% ). Strong cytotoxic effect was recorded for tansy and yarrow extracts (125-1000 µg/mL) by xCELLigence system and MTS assay. Conversely, a supportive effect on cell proliferation was recorded for goldenrod extracts (125 µg/mL) by the same methods ( < 0.001). The antioxidant activity was in good correlation with total polyphenolic content, and the highest value was recorded for goldenrod leaves, followed by tansy leaves, goldenrod flowers and yarrow leaf extracts. The goldenrod extracts were abundant with flavonoids, whereas phenolic acid derivatives predominated in the polyphenolic profile of tansy and yarrow.
菊科植物因其在治疗胃肠道疾病方面的疗效而被广泛应用，但过量摄入的后果仍需研究。本研究的目的是评价 L.（艾菊）、L.（蓍草）和 Ait.（一枝黄花）的乙醇提取物的细胞毒性、抗氧化性能和测定多酚谱。通过使用猪肠上皮细胞系（IPEC-1）和通过测量代谢活性的变化（（3-(4,5-二甲基噻唑-2-基)-5-(3-羧甲氧基苯基)-2-(4-磺基苯基)-2H-四唑）（MTS）测定法），通过实时 xCELLigence 系统监测提取物的细胞毒性。通过分光光度法测定抗氧化性能，并用 HPLC-DAD 测定 50%乙醇提取物（10%）的多酚谱。通过 xCELLigence 系统和 MTS 测定法，记录到艾菊和蓍草提取物（125-1000 µg/mL）具有很强的细胞毒性作用。相反，通过相同的方法记录到一枝黄花提取物（125 µg/mL）对细胞增殖有支持作用（<0.001）。抗氧化活性与总多酚含量密切相关，一枝黄花叶提取物的抗氧化活性最高，其次是艾菊叶提取物、一枝黄花花提取物和蓍草叶提取物。一枝黄花提取物富含类黄酮，而艾菊和蓍草的多酚谱中则以酚酸衍生物为主。
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