Klurfeld D M
Arch Pathol Lab Med. 1985 May;109(5):445-9.
Four monoclonal antibodies against human monocyte/macrophage epitopes were used in an immunoperoxidase procedure to identify foam cells in atherosclerotic plaques from human aortas and coronary arteries. All antibodies gave positive reactions with the majority of foam cells observed in fatty streaks and advanced atheromas. Immunoperoxidase staining correlated with oil red O and nonspecific esterase-positive areas. Use of the immunohistochemical technique on Bouin's-fixed, paraffin-embedded tissue gave good results that afforded better localization and cell morphology than observed with frozen sections. In addition to the identification of the majority of foam cells as monocytic in origin, the technique described allowed visualization of membrane-bound extracellular droplets that were monocyte derived within necrotic cores of atheromas, indicating a contribution by dead macrophages to the lipid of atherosclerotic plaques.
四种针对人单核细胞/巨噬细胞表位的单克隆抗体用于免疫过氧化物酶法，以鉴定来自人主动脉和冠状动脉的动脉粥样硬化斑块中的泡沫细胞。所有抗体对在脂肪条纹和晚期动脉粥样硬化中观察到的大多数泡沫细胞均呈阳性反应。免疫过氧化物酶染色与油红O和非特异性酯酶阳性区域相关。在经Bouin固定、石蜡包埋的组织上使用免疫组织化学技术取得了良好的效果，与冰冻切片相比，能提供更好的定位和细胞形态。除了鉴定出大多数泡沫细胞起源于单核细胞外，所述技术还能观察到动脉粥样硬化坏死核心内源自单核细胞的膜结合细胞外液滴，表明死亡的巨噬细胞对动脉粥样硬化斑块脂质有贡献。
