Sokolov Igor, Guz Natali V, Iyer Swaminathan, Hewitt Amy, Sokolov Nina A, Erlichman Joseph S, Woodworth Craig D
Departments of Mechanical Engineering, Biomedical Engineering, Physics, Tufts University, Medford, Massachusetts, United States of America.
Department of Chemistry, Clarkson University, Potsdam, New York, United States of America.
PLoS One. 2015 Mar 25;10(3):e0122774. doi: 10.1371/journal.pone.0122774. eCollection 2015.
The size increase of skin epithelial cells during aging is well-known. Here we demonstrate that treatment of aging cells with cytochalasin B substantially decreases cell size. This decrease was demonstrated on a mouse model and on human skin cells in vitro. Six nude mice were treated by topical application of cytochalasin B on skin of the dorsal left midsection for 140 days (the right side served as control for placebo treatment). An average decrease in cell size of 56±16% resulted. A reduction of cell size was also observed on primary human skin epithelial cells of different in vitro age (passages from 1 to 8). A cell strain obtained from a pool of 6 human subjects was treated with cytochalasin B in vitro for 12 hours. We observed a decrease in cell size that became statistically significant and reached 20-40% for cells of older passage (6-8 passages) whereas no substantial change was observed for younger cells. These results may be important for understanding the aging processes, and for cosmetic treatment of aging skin.
皮肤上皮细胞在衰老过程中体积增大是众所周知的。在此我们证明，用细胞松弛素B处理衰老细胞可显著减小细胞体积。这种减小在小鼠模型和体外培养的人皮肤细胞中均得到了证实。六只裸鼠，通过在左侧腹部背部皮肤局部涂抹细胞松弛素B进行处理，持续140天（右侧作为安慰剂处理的对照）。细胞体积平均减小了56±16%。在不同体外传代年龄（1至8代）的原代人皮肤上皮细胞中也观察到了细胞体积的减小。从6名人类受试者的细胞池中获得的一个细胞株在体外经细胞松弛素B处理12小时。我们观察到细胞体积减小，对于传代次数较多（6 - 8代）的细胞，这种减小具有统计学意义，达到了20 - 40%，而对于传代次数较少的细胞则未观察到明显变化。这些结果对于理解衰老过程以及衰老皮肤的美容治疗可能具有重要意义。
