Aroca Ángeles, Serna Antonio, Gotor Cecilia, Romero Luis C
Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas y Universidad de Sevilla, 41092 Seville, Spain (A.A., C.G., L.C.R.); andAB Sciex, 28108 Alcobendas, Madrid, Spain (A.S.).
Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas y Universidad de Sevilla, 41092 Seville, Spain (A.A., C.G., L.C.R.); andAB Sciex, 28108 Alcobendas, Madrid, Spain (A.S.)
Plant Physiol. 2015 May;168(1):334-42. doi: 10.1104/pp.15.00009. Epub 2015 Mar 25.
Hydrogen sulfide is a highly reactive molecule that is currently accepted as a signaling compound. This molecule is as important as carbon monoxide in mammals and hydrogen peroxide in plants, as well as nitric oxide in both eukaryotic systems. Although many studies have been conducted on the physiological effects of hydrogen sulfide, the underlying mechanisms are poorly understood. One of the proposed mechanisms involves the posttranslational modification of protein cysteine residues, a process called S-sulfhydration. In this work, a modified biotin switch method was used for the detection of Arabidopsis (Arabidopsis thaliana) proteins modified by S-sulfhydration under physiological conditions. The presence of an S-sulfhydration-modified cysteine residue on cytosolic ascorbate peroxidase was demonstrated using liquid chromatography-tandem mass spectrometry analysis, and a total of 106 S-sulfhydrated proteins were identified. Immunoblot and enzyme activity analyses of some of these proteins showed that the sulfide added through S-sulfhydration reversibly regulates the functions of plant proteins in a manner similar to that described in mammalian systems.
硫化氢是一种高反应性分子，目前被公认为一种信号化合物。该分子在哺乳动物中与一氧化碳、在植物中与过氧化氢以及在两个真核系统中与一氧化氮同样重要。尽管已经对硫化氢的生理效应进行了许多研究，但其潜在机制仍知之甚少。一种提出的机制涉及蛋白质半胱氨酸残基的翻译后修饰，这一过程称为S-硫水合作用。在这项工作中，使用一种改良的生物素开关法来检测生理条件下经S-硫水合作用修饰的拟南芥蛋白。利用液相色谱-串联质谱分析证明了胞质抗坏血酸过氧化物酶上存在一个经S-硫水合作用修饰的半胱氨酸残基，共鉴定出106种经S-硫水合作用修饰的蛋白。对其中一些蛋白的免疫印迹和酶活性分析表明，通过S-硫水合作用添加的硫化物以类似于哺乳动物系统中所描述的方式可逆地调节植物蛋白的功能。
