Charles-de-Sá Luiz, Gontijo-de-Amorim Natale Ferreira, Maeda Takiya Christina, Borojevic Radovan, Benati Donatella, Bernardi Paolo, Sbarbati Andrea, Rigotti Gino
Verona, Italy; and Rio de Janeiro, Brazil From the Department of Neurological and Motor Science, Section of Anatomy and Histology, University of Verona; the Unità di Chirurgia Rigenerativa, Clinica San Francesco; and the Postgraduate Program in Surgical Science, Federal University of Rio de Janeiro.
Plast Reconstr Surg. 2015 Apr;135(4):999-1009. doi: 10.1097/PRS.0000000000001123.
The regenerative property of fat grafting has been described. However, it is not clear whether the clinical results are attributable to the stem cells or are linked to other components of the adipose tissue. This work is aimed at analysis of the histologic and ultrastructural changes of aged facial skin after injection of fat graft in addition to its stromal vascular fraction, obtained by centrifugation, and to compare the results with those obtained by the injection of expanded adipose-derived mesenchymal stem cells.
This study was performed in six consecutive patients who were candidates for face lift and whose ages ranged between 45 and 65 years. The patients underwent sampling of fat by liposuction from the abdominal region. The injection of fat and its stromal vascular fraction or expanded mesenchymal stem cells was performed in the preauricular areas. Fragments of skin were removed before and 3 months after each treatment and analyzed by optical and electron microscopy.
After treatment with the autologous lipidic component and stromal vascular fraction, the skin showed a decrease in elastic fiber network (elastosis) and the appearance of new oxytalan elastic fibers in papillary dermis. The ultrastructural examination showed a modified tridimensional architecture of the reticular dermis and the presence of a richer microvascular bed. Similar results following treatment with expanded mesenchymal stem cells were observed.
This study demonstrates that treatment with either fat and stromal vascular fraction or expanded mesenchymal stem cells modifies the pattern of the dermis, representing a skin rejuvenation effect.
脂肪移植的再生特性已被描述。然而，尚不清楚临床效果是归因于干细胞还是与脂肪组织的其他成分有关。这项工作旨在分析注射脂肪移植及其通过离心获得的基质血管成分后老年面部皮肤的组织学和超微结构变化，并将结果与注射扩增的脂肪来源间充质干细胞所获得的结果进行比较。
本研究对6例连续的年龄在45至65岁之间、适合进行面部提升的患者进行。患者通过吸脂术从腹部区域采集脂肪。在耳前区域注射脂肪及其基质血管成分或扩增的间充质干细胞。在每次治疗前和治疗后3个月取皮肤碎片，通过光学显微镜和电子显微镜进行分析。
用自体脂质成分和基质血管成分治疗后，皮肤显示弹性纤维网络减少（弹性组织变性），乳头真皮中出现新的弹力纤维。超微结构检查显示网状真皮的三维结构改变，微血管床更丰富。用扩增的间充质干细胞治疗后观察到类似结果。
本研究表明，用脂肪和基质血管成分或扩增的间充质干细胞治疗可改变真皮模式，呈现皮肤年轻化效果。
