Plestina-Borjan Ivna, Katusic Damir, Medvidovic-Grubisic Maria, Supe-Domic Daniela, Bucan Kajo, Tandara Leida, Rogosic Veljko
Department of Ophthalmology, University of Split School of Medicine, Spinciceva 1, 21000 Split, Croatia.
Department of Ophthalmology, University of Zagreb School of Medicine, Salata 3, 10000 Zagreb, Croatia.
Oxid Med Cell Longev. 2015;2015:804054. doi: 10.1155/2015/804054. Epub 2015 Feb 28.
The aim was to estimate association of the oxidative stress with the occurrence of age-related macular degeneration (AMD). The activities of erythrocyte antioxidant enzymes: superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) and additionally serum total antioxidant status (TAS) were used as indicators of the oxidative stress level. 57 AMD patients (32 early and 25 late AMD) and 50 healthy, age and gender matched controls were included. GPx activity (P < 0.001) and serum TAS (P = 0.015) were significantly lower in AMD patients. The difference was not significant for SOD or CAT activities. Significant interaction between GPx and SOD was detected (P = 0.003). At high levels of SOD activity (over 75th percentile), one standard deviation decrease in GPx increases the odds for AMD for six times (OR = 6.22; P < 0.001). ROC analysis revealed that combined values of GPx activity and TAS are significant determinants of AMD status. Accuracy, sensitivity, specificity, and positive and negative predictive values were 75%, 95%, 52%, 69%, and 90%, respectively. The study showed that low GPx activity and TAS are associated with AMD. SOD modulates the association of GPx and AMD. The results suggest that erythrocyte antioxidant enzymes activity and serum TAS could be promising markers for the prediction of AMD.
目的是评估氧化应激与年龄相关性黄斑变性（AMD）发生之间的关联。红细胞抗氧化酶超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（CAT）的活性，以及血清总抗氧化状态（TAS）被用作氧化应激水平的指标。纳入了57例AMD患者（32例早期AMD和25例晚期AMD）以及50名年龄和性别匹配的健康对照者。AMD患者的GPx活性（P < 0.001）和血清TAS（P = 0.015）显著降低。SOD或CAT活性的差异不显著。检测到GPx和SOD之间存在显著交互作用（P = 0.003）。在SOD活性高水平（超过第75百分位数）时，GPx活性每降低一个标准差，AMD的患病几率增加6倍（OR = 6.22；P < 0.001）。ROC分析显示，GPx活性和TAS的综合值是AMD状态的重要决定因素。准确性、敏感性、特异性以及阳性和阴性预测值分别为75%、95%、52%、69%和90%。该研究表明，低GPx活性和TAS与AMD相关。SOD调节GPx与AMD之间的关联。结果表明，红细胞抗氧化酶活性和血清TAS可能是预测AMD的有前景的标志物。
