Čolak Emina, Žorić Lepša, Radosavljević Aleksandra, Ignjatović Svetlana
a Institute of Medical Biochemistry, Clinical Center of Serbia, and School of Pharmacy , University of Belgrade , Belgrade , Serbia.
b Clinic for Eye Diseases, Clinical Center, Faculty of Medicine , University of Pristina, Settlement Kosovska Mitrovica , Serbia.
Curr Eye Res. 2018 May;43(5):659-665. doi: 10.1080/02713683.2018.1437452. Epub 2018 Feb 15.
Age-related macular degeneration (AMD) is the leading cause of the irreversible central visual loss among the elderly in the developed countries. Iron is considered a potent generator of the oxidative damage whose levels increase with age, potentially exacerbating the age-related diseases. The aim of this study was to assess the serum values of iron, and iron-binding proteins (transferrin, ferritin, and haptoglobin) in patients with AMD along with the parameters of the antioxidant defense: superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase, and total antioxidant status (TAS), in order to analyze the possible impact of iron and iron-binding proteins to the development of oxidative stress in AMD patients, and the association of the selected parameters with the AMD. In addition, the aim was to examine the gender differences and calculate the cutoff points of tested parameters that could be associated with AMD.
A cross-sectional study included 55 AMD patients aged 71.7 ± 7.36 years and 65 aged-matched control subjects aged 70.25 ± 6.46 years.
Significantly lower ferritin (P = 0.025), SOD (P = 0.026), GPx (P = 0.019), and TAS (P < 0.004) values were found in patients with AMD compared to the controls (P < 0.05). Significant association of GPx < 27 U/gHb (odds ratio [OR]: 1.13; 95% confidence interval [CI] 0.78-2.10; P = 0.049), TAS < 1.25 mmol/L (OR: 5.77; 95% CI 0.98-367.0; P < 0.000), ferritin < 84.8 pg/mL (OR: 2.52; 95% CI 1.37-4.62; P = 0.002), and haptoglobin<1.51 g/L (OR: 1.94; 95% CI 1.05-3.56; P = 0.031) was found with the AMD. According to receiver operating characteristic curve analysis, ferritin concentration <84.8 pg/L, GPx < 27 U/gHb, and TAS < 1.25 mmol/L have sufficient predictive ability for AMD.
Significantly reduced capacity of the antioxidant defense system and iron-binding storage proteins (ferritin) found in AMD could have an important role in the development of increase oxidative stress in AMD patients.
年龄相关性黄斑变性（AMD）是发达国家老年人不可逆性中心视力丧失的主要原因。铁被认为是氧化损伤的强效诱导剂，其水平随年龄增长而升高，可能会加剧与年龄相关的疾病。本研究的目的是评估AMD患者血清中铁及铁结合蛋白（转铁蛋白、铁蛋白和触珠蛋白）的值，以及抗氧化防御参数：超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽还原酶和总抗氧化状态（TAS），以分析铁和铁结合蛋白对AMD患者氧化应激发展的可能影响，以及所选参数与AMD的关联。此外，目的是研究性别差异，并计算可能与AMD相关的测试参数的截断点。
一项横断面研究纳入了55例年龄为71.7±7.36岁的AMD患者和65例年龄匹配的对照者，年龄为70.25±6.46岁。
与对照组相比，AMD患者的铁蛋白（P = 0.025）、SOD（P = 0.026）、GPx（P = 0.019）和TAS（P < 0.004）值显著降低（P < 0.05）。发现GPx < 27 U/gHb（比值比[OR]：1.13；95%置信区间[CI] 0.78 - 2.10；P = 0.049）、TAS < 1.25 mmol/L（OR：5.77；95% CI 0.98 - 367.0；P < 0.000）、铁蛋白 < 84.8 pg/mL（OR：2.52；95% CI 1.37 - 4.62；P = 0.002）和触珠蛋白<1.51 g/L（OR：1.94；95% CI 1.05 - 3.56；P = 0.031）与AMD显著相关。根据受试者工作特征曲线分析，铁蛋白浓度<84.8 pg/L、GPx < 27 U/gHb和TAS < 1.25 mmol/L对AMD具有足够的预测能力。
在AMD患者中发现抗氧化防御系统和铁结合储存蛋白（铁蛋白）的能力显著降低，这可能在AMD患者氧化应激增加的发展中起重要作用。
