Nagai Takeshi, Tanoue Yasuhiro, Kai Norihisa, Suzuki Nobutaka
Graduate School of Agricultural Sciences, Yamagata University, Tsuruoka, Yamagata 9978555 Japan ; The United Graduate School of Agricultural Sciences, Iwate University, Morioka, Iwate 0208550 Japan ; Graduate School, Prince of Songkla University, Songkhla, 90112 Thailand.
National Fisheries University, Shimonoseki, Yamaguchi 7596595 Japan.
J Food Sci Technol. 2015 Apr;52(4):2344-51. doi: 10.1007/s13197-014-1266-1. Epub 2014 Apr 24.
Collagen from emu skins as a by-product was prepared. The skins were hardly solubilized in acetic acid, however were successfully solubilized on digestion with 10 % pepsin (w/w) for 4 days. The yield of pepsin-solubilized collagen (PSC) was about 27.3 %, on a raw weight basis. By SDS-PAGE and CM-Toyopearl 650 M column chromatography, the presence of a fourth subunit that was previously designated α4 was confirmed. The denaturation temperature of the PSC was 31.5 °C, about 6-7 °C lower than that from the porcine skins. ATR-FTIR analysis indicated that the helical arrangements of the PSC from emu skins existed and its structures of PSC were changed slightly due to the loss of N- and C-terminus domains in similar to that from the porcine skins. That is, the PSC from emu skins did not possess telopeptide chains as major portion of antigenic sites in collagen. The present study indicates that a large quantity of emu skins as by-products have potential as a good alternative source of high-quality collagen for industrial purposes in the foods, cosmetics, and pharmaceutical and biomedical fields.
制备了来自鸸鹋皮的胶原蛋白作为副产品。这些皮在乙酸中几乎不溶解，然而在用10%（w/w）胃蛋白酶消化4天后成功溶解。以原始重量计算，胃蛋白酶溶解胶原蛋白（PSC）的产率约为27.3%。通过SDS-PAGE和CM-Toyopearl 650M柱色谱法，证实了先前命名为α4的第四个亚基的存在。PSC的变性温度为31.5℃，比猪皮中的低约6-7℃。ATR-FTIR分析表明，鸸鹋皮PSC存在螺旋结构，并且由于N端和C端结构域的缺失，其结构与猪皮中的类似，略有变化。也就是说，鸸鹋皮PSC不具有作为胶原蛋白中主要抗原位点的端肽链。本研究表明，大量作为副产品的鸸鹋皮有潜力成为食品、化妆品、制药和生物医学领域工业用途中优质胶原蛋白的良好替代来源。
