Phulara Suresh C, Shukla Virendra, Tiwari Sudeep, Pandey Rakesh
Department of Microbial Technology and Nematology, CSIR - Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, India.
Pharmacogn Mag. 2015 Apr-Jun;11(42):410-6. doi: 10.4103/0973-1296.153097.
Bacopa monnieri (L.) Pennell, commonly known as Brahmi is an important medicinal plant traditionally used as memory enhancer and antiepileptic agent.
The present study investigated antioxidant and stress resistance potentials of B. monnieri aqueous extract (BMW) using Caenorhabditis elegans animal model system.
The antioxidant activity of the BMW was measured using in vitro (DPPH, reducing power and total polyphenol content) and in vivo (DCF-DA assay) assays. The antistress potential of BMW (0.1, 0.01, and 0.001 mg/ml) was evaluated through thermal stress (37°C) and oxidative stress (10 mM paraquat) using C. elegans. Quantification of the HSP-16.2 level was done using CL2070 transgenic worms.
Present study reveals that BMW possess in vitro and in vivo antioxidant activities. BMW significantly enhanced stress tolerance and increased the mean lifespan of worms during thermal and oxidative stress, although it did not extend lifespan at 20°C and attenuated age dependent decline in physiological behaviors. Moreover, it was shown that BMW was able to up-regulate expression of stress associated gene hsp-16.2, which significantly (P < 0.001) extends the mean lifespan of worms under stress conditions.
The study strongly suggests that BMW acts as an antistressor and potent reactive oxygen species scavenger which enhances the survival of the worms in different stress conditions.
假马齿苋（Bacopa monnieri (L.) Pennell），俗称婆罗门参，是一种重要的药用植物，传统上用作记忆增强剂和抗癫痫剂。
本研究使用秀丽隐杆线虫动物模型系统研究假马齿苋水提取物（BMW）的抗氧化和抗应激潜力。
使用体外（DPPH、还原力和总多酚含量）和体内（DCF-DA测定）试验测量BMW的抗氧化活性。通过使用秀丽隐杆线虫的热应激（37°C）和氧化应激（10 mM百草枯）评估BMW（0.1、0.01和0.001 mg/ml）的抗应激潜力。使用CL2070转基因蠕虫对HSP-16.2水平进行定量。
本研究表明，BMW具有体外和体内抗氧化活性。BMW显著增强了应激耐受性，并在热应激和氧化应激期间延长了蠕虫的平均寿命，尽管它在20°C时没有延长寿命，并且减弱了生理行为的年龄依赖性下降。此外，研究表明，BMW能够上调应激相关基因hsp-·16.2的表达，这在应激条件下显著（P < 0.001）延长了蠕虫的平均寿命。
该研究强烈表明，BMW作为一种抗应激剂和有效的活性氧清除剂，可提高蠕虫在不同应激条件下的存活率。
