Sae-Tan Sudathip, Rogers Connie J, Lambert Joshua D
Department of Food Science, The Pennsylvania State University, University Park, PA 16802.
Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA 16802.
J Funct Foods. 2015 Apr 1;14:210-214. doi: 10.1016/j.jff.2015.01.036.
We have previously reported that decaffeinated green tea extract (GTE) in combination with voluntary exercise (Ex) reduces metabolic syndrome in high fat-fed C57BL/6J mice. Here, we examined for the first time the effect of treatment with 77 mg/g GTE, Ex, or both (GTE + Ex) on genes related to the conversion of white adipose tissue (WAT) to brown fat-like adipose tissue (BLAT) in this model. GTE+Ex induced genes related to lipolysis (hormone sensitive lipase [3.0-fold] and patatin-like phospholipase domain-containing protein 2 [2-fold]), mitochondrial β-oxidation (NADH dehydrogenase 5 [2.3-fold], cytochrome B [2.0-fold], and cytochrome C oxidase III [1.9-fold increase]), and adipose tissue browning (peroxisome proliferator-activated receptor-γ coactivator-1α [1.8-fold], bone morphogenetic protein 4 [2.6-fold], and phosphatase and tensin homolog [2.6-fold]) in visceral WAT compared to HF-fed mice. These results suggest that GTE+Ex function in part by inducing the conversion of WAT to BLAT and provides novel mechanistic insight into this combination.
我们之前报道过，脱咖啡因绿茶提取物（GTE）与自主运动（Ex）相结合可减轻高脂喂养的C57BL/6J小鼠的代谢综合征。在此，我们首次研究了在该模型中，用77 mg/g GTE、Ex或两者联合（GTE + Ex）处理对与白色脂肪组织（WAT）向棕色脂肪样脂肪组织（BLAT）转化相关基因的影响。与高脂喂养的小鼠相比，GTE + Ex诱导了内脏WAT中与脂肪分解（激素敏感性脂肪酶[3.0倍]和含patatin样磷脂酶结构域蛋白2[2倍]）、线粒体β-氧化（NADH脱氢酶5[2.3倍]、细胞色素B[2.0倍]和细胞色素C氧化酶III[增加1.9倍]）以及脂肪组织褐变（过氧化物酶体增殖物激活受体γ共激活因子-1α[1.8倍]、骨形态发生蛋白4[2.6倍]和磷酸酶及张力蛋白同源物[2.6倍]）相关的基因。这些结果表明，GTE + Ex部分通过诱导WAT向BLAT的转化发挥作用，并为这种联合作用提供了新的机制见解。
