Matena Julia, Petersen Svea, Gieseke Matthias, Kampmann Andreas, Teske Michael, Beyerbach Martin, Murua Escobar Hugo, Haferkamp Heinz, Gellrich Nils-Claudius, Nolte Ingo
Small Animal Clinic, University of Veterinary Medicine Hannover, Foundation, D-30559 Hannover, Germany.
Institute for Biomedical Engineering, Rostock University Medical Center, D-18119 Rostock, Germany.
Int J Mol Sci. 2015 Apr 2;16(4):7478-92. doi: 10.3390/ijms16047478.
To improve well-known titanium implants, pores can be used for increasing bone formation and close bone-implant interface. Selective Laser Melting (SLM) enables the production of any geometry and was used for implant production with 250-µm pore size. The used pore size supports vessel ingrowth, as bone formation is strongly dependent on fast vascularization. Additionally, proangiogenic factors promote implant vascularization. To functionalize the titanium with proangiogenic factors, polycaprolactone (PCL) coating can be used. The following proangiogenic factors were examined: vascular endothelial growth factor (VEGF), high mobility group box 1 (HMGB1) and chemokine (C-X-C motif) ligand 12 (CXCL12). As different surfaces lead to different cell reactions, titanium and PCL coating were compared. The growing into the porous titanium structure of primary osteoblasts was examined by cross sections. Primary osteoblasts seeded on the different surfaces were compared using Live Cell Imaging (LCI). Cross sections showed cells had proliferated, but not migrated after seven days. Although the cell count was lower on titanium PCL implants in LCI, the cell count and cell spreading area development showed promising results for titanium PCL implants. HMGB1 showed the highest migration capacity for stimulating the endothelial cell line. Future perspective would be the incorporation of HMGB1 into PCL polymer for the realization of a slow factor release.
为改进知名的钛植入物，可利用孔隙来增加骨形成并缩小骨 - 植入物界面。选择性激光熔化（SLM）能够制造任何几何形状，被用于生产孔径为250微米的植入物。所使用的孔径支持血管长入，因为骨形成强烈依赖于快速血管化。此外，促血管生成因子可促进植入物的血管化。为用促血管生成因子使钛功能化，可使用聚己内酯（PCL）涂层。对以下促血管生成因子进行了检测：血管内皮生长因子（VEGF）、高迁移率族蛋白B1（HMGB1）和趋化因子（C - X - C基序）配体12（CXCL12）。由于不同表面会导致不同的细胞反应，因此对钛和PCL涂层进行了比较。通过横截面检查原代成骨细胞向多孔钛结构中的生长情况。使用活细胞成像（LCI）比较接种在不同表面上的原代成骨细胞。横截面显示细胞在七天后已增殖但未迁移。尽管在LCI中钛PCL植入物上的细胞计数较低，但细胞计数和细胞铺展面积的发展对钛PCL植入物显示出有前景的结果。HMGB1在刺激内皮细胞系方面显示出最高的迁移能力。未来的展望将是把HMGB1掺入PCL聚合物中以实现因子的缓慢释放。
