Wang Zhen, Bie Peng, Cheng Jie, Wu Qing, Lu Lin
Ann Clin Microbiol Antimicrob. 2015 Mar 21;14:16. doi: 10.1186/s12941-015-0077-1.
Brucellosis is a zoonosis that disseminated by a variety of ways between animals and humans. The effective disinfection of contaminated environments, soil, feces, and animal bodies plays an irreplaceable role in the prevention and control of brucellosis. To kill Brucella effectively, the bactericidal effects of frequently used disinfectants (including aldehydes, halogens, quaternary ammonium compound, phenolics, and alkalines) and the potential factors that influence disinfection effects were determined in the present study. The results revealed that the minimum bactericidal concentrations (MBCs) of the six disinfectants were all significantly lower than the routinely used concentrations, and all the tested disinfectants were effective against B. melitensis NI. The results of quantitative determination showed that the bactericidal effects of the disinfectants were influenced by their concentration, exposure time, dirty condition and the temperature. Under dirty conditions and a low temperatures, sodium hypochlorite and sodium hydroxide showed better bactericidal effect, while benzalkonium chloride was almost without bactericidal ability. In addition, increasing the disinfectant concentration at low temperatures can improve the bactericidal effect. The present study suggested that Brucella is sensitive to commonly used disinfectants. However, the bactericidal effect is vulnerable to dirty conditions and low temperatures. Thus, it is necessary to test the in vitro sensitivity of disinfectants that are commonly used on farms or the new disinfectant formulations periodically, with the aim of improving the efficacy of animal and human brucellosis prevention programs.
布鲁氏菌病是一种人畜共患病，可通过多种方式在动物和人类之间传播。对受污染的环境、土壤、粪便和动物尸体进行有效消毒在布鲁氏菌病的防控中发挥着不可替代的作用。为有效杀灭布鲁氏菌，本研究测定了常用消毒剂（包括醛类、卤素类、季铵化合物、酚类和碱类）的杀菌效果以及影响消毒效果的潜在因素。结果表明，六种消毒剂的最低杀菌浓度均显著低于常规使用浓度，且所有受试消毒剂对羊种布鲁氏菌NI均有效。定量测定结果表明，消毒剂的杀菌效果受其浓度、作用时间、污染状况和温度的影响。在污染状况和低温条件下，次氯酸钠和氢氧化钠表现出较好的杀菌效果，而苯扎氯铵几乎没有杀菌能力。此外，在低温下提高消毒剂浓度可增强杀菌效果。本研究表明，布鲁氏菌对常用消毒剂敏感。然而，杀菌效果易受污染状况和低温的影响。因此，有必要定期检测养殖场常用消毒剂或新型消毒剂配方的体外敏感性，以提高动物和人类布鲁氏菌病预防计划的效果。
