致病性及多重耐药性艰难梭菌参考菌株630的全基因组重测序
Genome Resequencing of the Virulent and Multidrug-Resistant Reference Strain Clostridium difficile 630.
作者信息
Riedel Thomas, Bunk Boyke, Thürmer Andrea, Spröer Cathrin, Brzuszkiewicz Elzbieta, Abt Birte, Gronow Sabine, Liesegang Heiko, Daniel Rolf, Overmann Jörg
机构信息
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany
Department of Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Georg-August-University Göttingen, Göttingen, Germany.
出版信息
Genome Announc. 2015 Apr 9;3(2):e00276-15. doi: 10.1128/genomeA.00276-15.
Abstract
We resequenced the complete genome of the virulent and multidrug-resistant pathogen Clostridium difficile strain 630. A combination of single-molecule real-time and Illumina sequencing technology revealed the presence of an additional rRNA gene cluster, additional tRNAs, and the absence of a transposon in comparison to the published and reannotated genome sequence.
摘要
我们对强毒力且多重耐药的艰难梭菌菌株630的全基因组进行了重测序。与已发表并重新注释的基因组序列相比，单分子实时测序和Illumina测序技术相结合揭示了额外的rRNA基因簇、额外的tRNA的存在，以及转座子的缺失。
相似文献
1
Genome Resequencing of the Virulent and Multidrug-Resistant Reference Strain Clostridium difficile 630.致病性及多重耐药性艰难梭菌参考菌株630的全基因组重测序
Genome Announc. 2015 Apr 9;3(2):e00276-15. doi: 10.1128/genomeA.00276-15.
2
Manual curation and reannotation of the genomes of Clostridium difficile 630Δerm and C. difficile 630.艰难梭菌630Δerm和艰难梭菌630基因组的人工整理与重新注释。
J Med Microbiol. 2017 Mar;66(3):286-293. doi: 10.1099/jmm.0.000427.
3
Genomic analyses of multidrug resistant Pseudomonas aeruginosa PA1 resequenced by single-molecule real-time sequencing.通过单分子实时测序对多重耐药铜绿假单胞菌PA1进行的基因组分析。
Biosci Rep. 2016 Nov 29;36(6). doi: 10.1042/BSR20160282. Print 2016 Dec.
4
Complete Genome Sequence of the Clostridium difficile Type Strain DSM 1296T.艰难梭菌模式菌株DSM 1296T的全基因组序列
Genome Announc. 2015 Oct 8;3(5):e01186-15. doi: 10.1128/genomeA.01186-15.
5
Molecular analysis of three Clostridium difficile strain genomes isolated from pig farm-related samples.从猪场相关样本中分离出的三株艰难梭菌菌株基因组的分子分析。
Anaerobe. 2017 Dec;48:224-231. doi: 10.1016/j.anaerobe.2017.09.002. Epub 2017 Sep 18.
6
The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome.多重耐药的人类病原体艰难梭菌拥有高度可移动的镶嵌基因组。
Nat Genet. 2006 Jul;38(7):779-86. doi: 10.1038/ng1830. Epub 2006 Jun 25.
7
Genome Sequence of E28, a Multidrug-Resistant Strain Isolated from a Chicken Carcass, and Its Spontaneously Inducible Prophage.从鸡 carcass 分离出的多药耐药菌株 E28 的基因组序列及其自发诱导性原噬菌体。 （注：carcass 常见释义为“屠体”“胴体”，这里翻译为“鸡 carcass”是因为原文未明确具体所指，保留英文更合适，若结合语境可灵活翻译为“鸡屠体”等）
Genome Announc. 2017 Jun 1;5(22):e00348-17. doi: 10.1128/genomeA.00348-17.
8
Independent Microevolution Mediated by Mobile Genetic Elements of Individual Clostridium difficile Isolates from Clade 4 Revealed by Whole-Genome Sequencing.全基因组测序揭示4型艰难梭菌分离株中移动遗传元件介导的独立微进化
mSystems. 2019 Mar 26;4(2). doi: 10.1128/mSystems.00252-18. eCollection 2019 Mar-Apr.
9
Complete genome sequence of the Clostridium difficile laboratory strain 630Δerm reveals differences from strain 630, including translocation of the mobile element CTn5.艰难梭菌实验室菌株630Δerm的全基因组序列揭示了其与菌株630的差异，包括移动元件CTn5的易位。
BMC Genomics. 2015 Jan 31;16(1):31. doi: 10.1186/s12864-015-1252-7.
10
Multidrug resistance in European Clostridium difficile clinical isolates.欧洲艰难梭菌临床分离株的多重耐药性。
J Antimicrob Chemother. 2011 Oct;66(10):2227-34. doi: 10.1093/jac/dkr292. Epub 2011 Jul 19.
引用本文的文献
1
Enabling next-generation anaerobic cultivation through biotechnology to advance functional microbiome research.通过生物技术实现下一代厌氧培养，以推进功能性微生物组研究。
Nat Biotechnol. 2025 Apr 29. doi: 10.1038/s41587-025-02660-6.
2
A Clostridioides difficile endolysin modulates toxin secretion without cell lysis.艰难梭菌溶细胞素调节毒素分泌而不导致细胞裂解。
Commun Biol. 2024 Aug 23;7(1):1044. doi: 10.1038/s42003-024-06730-4.
3
Removal of mobile genetic elements from the genome of and the implications for the organism's biology.从……基因组中去除可移动遗传元件及其对生物体生物学的影响。 （注：原文中“Removal of mobile genetic elements from the genome of ”这里“of”后面缺少具体内容）
Front Microbiol. 2024 Jun 20;15:1416665. doi: 10.3389/fmicb.2024.1416665. eCollection 2024.
4
MazEF Homologs in Exhibit Cross-Neutralization with Non-Cognate MazEFs.展示交叉中和的 MazEF 同源物与非同源 MazEF 之间的关系。
Toxins (Basel). 2024 Feb 3;16(2):81. doi: 10.3390/toxins16020081.
5
Role of the Spore Coat Proteins and , and the Spore Surface Protein CDIF630_02480, on the Surface Distribution of Exosporium Proteins in 630 Spores.芽孢衣蛋白以及芽孢表面蛋白CDIF630_02480在630型芽孢中外孢子蛋白表面分布上的作用
Microorganisms. 2022 Sep 27;10(10):1918. doi: 10.3390/microorganisms10101918.
6
A Highly Specific Holin-Mediated Mechanism Facilitates the Secretion of Lethal Toxin TcsL in .一种高度特异的 Holin 介导机制促进了致命毒素 TcsL 在. 中的分泌。
Toxins (Basel). 2022 Feb 8;14(2):124. doi: 10.3390/toxins14020124.
7
Predictive regulatory and metabolic network models for systems analysis of Clostridioides difficile.用于艰难梭菌系统分析的预测调控和代谢网络模型。
Cell Host Microbe. 2021 Nov 10;29(11):1709-1723.e5. doi: 10.1016/j.chom.2021.09.008. Epub 2021 Oct 11.
8
A novel hyperthermophilic methylglyoxal synthase: molecular dynamic analysis on the regional fluctuations.一种新型嗜热甲基乙二醛合酶：区域波动的分子动力学分析。
Sci Rep. 2021 Jan 28;11(1):2538. doi: 10.1038/s41598-021-82078-7.
9
Genetic Dissection of the Fermentative and Respiratory Contributions Supporting Vibrio cholerae Hypoxic Growth.遗传剖析支持霍乱弧菌缺氧生长的发酵和呼吸贡献。
J Bacteriol. 2020 Nov 19;202(24). doi: 10.1128/JB.00243-20.
10
Characterization of Clostridioides difficile DSM 101085 with A-B-CDT+ Phenotype from a Late Recurrent Colonization.从晚期复发性定植中具有 A-B-CDT+表型的艰难梭菌 DSM 101085 的特性。
Genome Biol Evol. 2020 May 1;12(5):566-577. doi: 10.1093/gbe/evaa072.
本文引用的文献
1
Complete genome sequence of the Clostridium difficile laboratory strain 630Δerm reveals differences from strain 630, including translocation of the mobile element CTn5.艰难梭菌实验室菌株630Δerm的全基因组序列揭示了其与菌株630的差异，包括移动元件CTn5的易位。
BMC Genomics. 2015 Jan 31;16(1):31. doi: 10.1186/s12864-015-1252-7.
2
Prokka: rapid prokaryotic genome annotation.Prokka：快速的原核生物基因组注释。
Bioinformatics. 2014 Jul 15;30(14):2068-9. doi: 10.1093/bioinformatics/btu153. Epub 2014 Mar 18.
3
Reannotation of the genome sequence of Clostridium difficile strain 630.艰难梭菌 630 株基因组序列的重新注释。
J Med Microbiol. 2011 Aug;60(Pt 8):1193-1199. doi: 10.1099/jmm.0.030452-0. Epub 2011 Feb 24.
4
Fast and accurate long-read alignment with Burrows-Wheeler transform.基于 Burrows-Wheeler 变换的快速准确长读比对。
Bioinformatics. 2010 Mar 1;26(5):589-95. doi: 10.1093/bioinformatics/btp698. Epub 2010 Jan 15.
5
The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome.多重耐药的人类病原体艰难梭菌拥有高度可移动的镶嵌基因组。
Nat Genet. 2006 Jul;38(7):779-86. doi: 10.1038/ng1830. Epub 2006 Jun 25.
6
Investigation of an outbreak of antibiotic-associated colitis by various typing methods.采用多种分型方法对抗生素相关性结肠炎暴发进行调查。
J Clin Microbiol. 1982 Dec;16(6):1096-101. doi: 10.1128/jcm.16.6.1096-1101.1982.
Zotero 插件