Havakhah Shahrzad, Sadeghnia Hamid R, Hajzadeh Mosa-Al-Reza, Roshan Nama Mohammadian, Shafiee Somayeh, Hosseinzadeh Hossein, Mohareri Narges, Rad Abolfazl Khajavi
Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran J Basic Med Sci. 2014 Dec;17(12):986-92.
There are a few previously reported studies about the effect of Nigella sativa oil on renal ischemia-reperfusion injury (IRI). The aim of the present study was to test the hypothesis whether pre- or post-treatment with N. sativa hydroalcoholic extract (NSE) would reduce tissue injury and oxidative damages in a clinically relevant rat model of renal IRI.
IRI was induced by clamping of bilateral renal arteries for 40 min fallowed by reperfusion for 180 min. NSE was prepared in a Soxhlet extractor and administrated with doses of 150 mg/kg or 300 mg/kg at 1 hr before ischemia induction (P-150 and 300) or at the beginning of reperfusion phase (T-150 and 300), via jugular catheter intravenously. The kidneys were then removed and subjected to biochemical analysis, comet assay or histopathological examination.
The kidneys of untreated IRI rats had a higher histopathological score (P<0.001), while in P-150, as well as T-150 and T-300 groups tubular lesions significantly decreased (P<0.001). Pre- and post-treatment with NSE also resulted in a significant decrease in malondialdehyde (MDA) level (P<0.001) and DNA damage (P<0.001) that were increased by renal I/R injury. NSE treatment also significantly restore (P<0.01) the decrease in renal thiol content caused by IRI.
The present study shows N. sativa extract has marked protective action against renal IRI, which may be partly due to its antioxidant effects.
此前有一些关于黑种草籽油对肾缺血再灌注损伤（IRI）影响的研究报道。本研究的目的是验证以下假设：在具有临床相关性的大鼠肾IRI模型中，黑种草水醇提取物（NSE）进行预处理或后处理是否会减轻组织损伤和氧化损伤。
通过夹闭双侧肾动脉40分钟，随后再灌注180分钟来诱导IRI。NSE在索氏提取器中制备，并在缺血诱导前1小时（P - 150和300）或再灌注阶段开始时（T - 150和300），经颈静脉导管静脉注射，剂量为150mg/kg或300mg/kg。然后取出肾脏进行生化分析、彗星试验或组织病理学检查。
未处理的IRI大鼠肾脏具有更高的组织病理学评分（P<0.001），而在P - 150组以及T - 150和T - 300组中，肾小管损伤显著减轻（P<0.001）。NSE预处理和后处理还导致丙二醛（MDA）水平（P<0.001）和DNA损伤（P<0.001）显著降低，这些指标因肾缺血/再灌注损伤而升高。NSE处理还显著恢复（P<0.01）了由IRI引起的肾硫醇含量降低。
本研究表明黑种草提取物对肾IRI具有显著的保护作用，这可能部分归因于其抗氧化作用。
