Sofka Dmitri, Pfeifer Agathe, Gleiss Barbara, Paulsen Peter, Hilbert Friederike
Berl Munch Tierarztl Wochenschr. 2015 Mar-Apr;128(3-4):104-10.
In most European countries human campylobacteriosis is the most important bacterial zoonotic foodborne infection. Chicken meat is considered the main source of infection. Since most strategies assessed so far, in reducing Campylobacter colonization in chickens or in the reduction of human disease, have not been very effective, new knowledge regarding Campylobacter's interaction with the host is needed. In this study we analysed fecal and cecal samples of five chicken flocks of different Austrian farms for the occurrence of Cjejuni and C. coli, and analysed the intestinal microbiota by PCR-SSCP, cultural detection of lactic acid bacteria, Enterococci, Staphylococci, Enterobacteriaceae, E. coli, and total aerobic colony counts. Furthermore ten chicken samples of cecal content of a flock during colonization with Campylobacter spp. was analysed by high throughput sequencing. With all three methods used we could detect a change within the microbiota caused by Cjejuni. Enumeration of different bacteria was significantly lower in fecal samples positive for C. jejuni, pointing out that a higher water content and thus, a preliminary stage of diarrhea might appear during Campylobacter colonization. By PCR-SSCP analysis the microbiota composition differed between colonized and non-colonized chicken fecal samples. This could also be detected in community analysis by high throughput sequencing, but this difference was only a tendency and not statistically significant. It can be concluded that C. jejuni is interacting with the intestinal microflora in their respective hosts and hence, this has to be taken into account when providing new strategies to combat Campylobacter colonization and disease.
在大多数欧洲国家，人弯曲杆菌病是最重要的细菌性食源性人畜共患病感染。鸡肉被认为是主要感染源。由于迄今为止评估的大多数减少鸡群中弯曲杆菌定植或减少人类疾病的策略效果都不太理想，因此需要有关弯曲杆菌与宿主相互作用的新知识。在本研究中，我们分析了奥地利不同农场的五个鸡群的粪便和盲肠样本中空肠弯曲杆菌和结肠弯曲杆菌的存在情况，并通过PCR-SSCP、乳酸菌、肠球菌、葡萄球菌、肠杆菌科、大肠杆菌的培养检测以及总需氧菌落计数来分析肠道微生物群。此外，对一个鸡群在弯曲杆菌属定植期间的十个盲肠内容物鸡样本进行了高通量测序分析。使用的所有三种方法都能检测到空肠弯曲杆菌引起的微生物群变化。空肠弯曲杆菌阳性粪便样本中不同细菌的计数明显较低，这表明在弯曲杆菌定植期间可能会出现较高的水分含量，从而出现腹泻的初期阶段。通过PCR-SSCP分析，定植和未定植鸡粪便样本的微生物群组成有所不同。在高通量测序的群落分析中也能检测到这种差异，但这种差异只是一种趋势，没有统计学意义。可以得出结论，空肠弯曲杆菌在其各自宿主中与肠道微生物群相互作用，因此，在提供对抗弯曲杆菌定植和疾病的新策略时必须考虑到这一点。
