Wang Meiyun, Hong Xiaohua, Chang Che-Feng, Li Qiang, Ma Bo, Zhang Hong, Xiang Sinan, Heo Hye-Young, Zhang Yi, Lee Dong-Hoon, Jiang Shanshan, Leigh Richard, Koehler Raymond C, van Zijl Peter C M, Wang Jian, Zhou Jinyuan
Division of MR Research, Department of Radiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Magn Reson Med. 2015 Jul;74(1):42-50. doi: 10.1002/mrm.25690. Epub 2015 Apr 16.
To explore the capability of amide proton transfer (APT) imaging in the detection of hemorrhagic and ischemic strokes using preclinical rat models.
The rat intracerebral hemorrhage (ICH) model (n = 10) was induced by injecting bacterial collagenase VII-S into the caudate nucleus, and the permanent ischemic stroke model (n = 10) was induced by using a 4-0 nylon suture to occlude the origin of the middle cerebral artery. APT-weighted (APTw) MRI was acquired on a 4.7T animal imager and quantified using the magnetization transfer-ratio asymmetry at 3.5 ppm from water.
There was a consistently high APTw MRI signal in hyperacute ICH during the initial 12 h after injection of collagenase compared with the contralateral brain tissue. When hemorrhagic and ischemic stroke were compared, hyperacute ICH and cerebral ischemia demonstrated opposite APTw MRI contrasts-namely, hyperintense versus hypointense compared with contralateral brain tissue, respectively. There was a stark contrast in APTw signal intensity between these two lesions.
APT-MRI could accurately detect hyperacute ICH and distinctly differentiate hyperacute ICH from cerebral ischemia, thus opening up the possibility of introducing to the clinic a single MRI scan for the simultaneous visualization and separation of hemorrhagic and ischemic strokes at the hyperacute stage. Magn Reson Med 74:42-50, 2015. © 2014 Wiley Periodicals, Inc.
利用临床前大鼠模型探讨酰胺质子转移（APT）成像在检测出血性和缺血性卒中方面的能力。
通过向尾状核注射细菌胶原酶VII-S诱导大鼠脑出血（ICH）模型（n = 10），并使用4-0尼龙缝线阻塞大脑中动脉起始部诱导永久性缺血性卒中模型（n = 10）。在4.7T动物成像仪上采集APT加权（APTw）MRI，并使用距水3.5 ppm处的磁化转移率不对称性进行定量分析。
与对侧脑组织相比，在注射胶原酶后的最初12小时内，超急性ICH区域的APTw MRI信号始终很高。当比较出血性和缺血性卒中时，超急性ICH和脑缺血表现出相反的APTw MRI对比——即与对侧脑组织相比，分别为高信号与低信号。这两种病变之间的APTw信号强度存在明显差异。
APT-MRI能够准确检测超急性ICH，并将其与脑缺血明显区分开来，从而为在临床上引入单次MRI扫描以同时可视化和区分超急性阶段的出血性和缺血性卒中开辟了可能性。《磁共振医学》74:42 - 50, 2015。© 2014威利期刊公司。
