Beckwith Kai Sandvold, Cooil Simon P, Wells Justin W, Sikorski Pawel
Department of Physics, Norwegian University of Science and Technology, Trondheim, Norway.
Nanoscale. 2015 May 14;7(18):8438-50. doi: 10.1039/c5nr00674k.
Nanoscale topographies and chemical patterns can be used as synthetic cell interfaces with a range of applications including the study and control of cellular processes. Herein, we describe the fabrication of high aspect ratio nanostructures using electron beam lithography in the epoxy-based polymer SU-8. We show how nanostructure geometry, position and fluorescence properties can be tuned, allowing flexible device design. Further, thiol-epoxide reactions were developed to give effective and specific modification of SU-8 surface chemistry. SU-8 nanostructures were made directly on glass cover slips, enabling the use of high resolution optical techniques such as live-cell confocal, total internal reflection and 3D structured illumination microscopy to investigate cell interactions with the nanostructures. Details of cell adherence and spreading, plasma membrane conformation and actin organization in response to high aspect ratio nanopillars and nanolines were investigated. The versatile structural and chemical properties combined with the high resolution cell imaging capabilities of this system are an important step towards the better understanding and control of cell interactions with nanomaterials.
纳米尺度的形貌和化学图案可作为合成细胞界面，具有一系列应用，包括细胞过程的研究和控制。在此，我们描述了使用电子束光刻技术在环氧基聚合物SU-8中制造高纵横比纳米结构的方法。我们展示了如何调整纳米结构的几何形状、位置和荧光特性，从而实现灵活的器件设计。此外，还开发了硫醇-环氧化合物反应，以对SU-8表面化学进行有效且特异的修饰。SU-8纳米结构直接在玻璃盖玻片上制成，使得能够使用高分辨率光学技术，如活细胞共聚焦、全内反射和三维结构照明显微镜，来研究细胞与纳米结构的相互作用。研究了细胞对高纵横比纳米柱和纳米线的粘附和铺展细节、质膜构象以及肌动蛋白组织。该系统的通用结构和化学性质与高分辨率细胞成像能力相结合，是朝着更好地理解和控制细胞与纳米材料相互作用迈出的重要一步。
