Liao Xiudong, Wu Rujuan, Ma Guang, Zhao Longmei, Zheng Zhaojun, Zhang Rijun
Laboratory of Feed Biotechnology, State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, People's Republic of China.
Lipids Health Dis. 2015 Apr 22;14:36. doi: 10.1186/s12944-015-0035-0.
Consumers are becoming increasingly interested in food containing high concentration of polyunsaturated fatty acids (PUFA). PUFA are considered as functional ingredients to prevent cardiovascular disease. The present study aimed to evaluate the effects of Clostridium butyricum on antioxidant properties, meat quality and fatty acid composition of broilers.
A total of 320 one-day-old Arbor Acres male chicks were randomly assigned to one of five treatments with eight replicates and fed a antibiotic-free basal corn-soybean meal diet (control) or the basal diet supplemented with either 2.5 × 10(8) (CB1), 5 × 10(8) (CB2) or 1 × 10(9) (CB3) cfu of C. butyricum/kg or 150 mg of aureomycin/kg (antibiotic) for 42 days.
The results showed that chicks fed diets supplemented with C. butyricum had higher (P < 0.05) superoxide dismutase activity and lower (P < 0.05) malondialdehyde concentration in liver compared with those in the control group. Broilers had lower (P < 0.05) cholesterol content of serum in either CB2 or CB3 treatment at day 21 and in the C. butyricum-supplemented groups at day 42 than those in the control group. Chicks fed CB3 diet had lower (P < 0.05) percentage of abdominal fat and higher (P < 0.05) breast muscle yield than those in the control and antibiotic groups. The supplementation of C. butyricum increased (P < 0.05) the concentrations of C20:1n-9, C20:2n-6, C20:3n-6, C20:3n-3, C20:4n-6, C20:5n-3, C22:6n-3 and total PUFA as well as ratio of PUFA to saturated fatty acids in breast muscle and the contents of C18:2 t-9, t-12, C20:3n-6, C20:3n-3 and C20:5n-3 in thigh muscle.
Supplementation of C. butyricum promotes hepatic antioxidant status, decreases cholesterol content of serum and percentage of abdominal fat, and improves meat quality and fatty acid composition of broiler birds. The results from the present study indicate that the increased PUFA concentrations in meat of broilers fed C. butyricum might be attributable to enhanced antioxidant activity.
消费者对含有高浓度多不饱和脂肪酸（PUFA）的食品越来越感兴趣。PUFA被认为是预防心血管疾病的功能性成分。本研究旨在评估丁酸梭菌对肉鸡抗氧化性能、肉质和脂肪酸组成的影响。
将320只1日龄的艾维茵雄性雏鸡随机分为5组，每组8个重复，分别饲喂不含抗生素的基础玉米-豆粕日粮（对照组）或添加2.5×10⁸（CB1）、5×10⁸（CB2）或1×10⁹（CB3）cfu/kg丁酸梭菌的基础日粮，或150mg/kg金霉素（抗生素）的日粮，持续42天。
结果表明，与对照组相比喂饲添加丁酸梭菌日粮的雏鸡肝脏中超氧化物歧化酶活性较高（P<0.05），丙二醛浓度较低（P<0.05）。在第21天，CB2或CB3处理组以及第42天添加丁酸梭菌的组的肉鸡血清胆固醇含量均低于对照组（P<0.05）。饲喂CB3日粮的雏鸡腹部脂肪百分比低于对照组和抗生素组（P<0.05），胸肌产量高于对照组和抗生素组（P<0.05）。添加丁酸梭菌增加了胸肌中C20:1n-9、C20:2n-6、C20:3n-6、C20:3n-3、C20:4n-6、C20:5n-3、C22:6n-3和总PUFA的浓度以及PUFA与饱和脂肪酸的比例，以及大腿肌肉中C18:2 t-9,t-12、C20:3n-6、C20:3n-3和C20:5n-3的含量（P<0.05）。
添加丁酸梭菌可促进肝脏抗氧化状态，降低血清胆固醇含量和腹部脂肪百分比，并改善肉鸡的肉质和脂肪酸组成。本研究结果表明，饲喂丁酸梭菌的肉鸡肌肉中PUFA浓度增加可能归因于抗氧化活性增强。
