Thongwat Damrongpan, Bunchu Nophawan
Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Muang, Phitsanulok 65000, Thailand; Centre of Excellence in Medical Biotechnology, Faculty of Medical Science, Naresuan University, Muang, Phitsanulok 65000, Thailand.
Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Muang, Phitsanulok 65000, Thailand; Centre of Excellence in Medical Biotechnology, Faculty of Medical Science, Naresuan University, Muang, Phitsanulok 65000, Thailand.
Asian Pac J Trop Med. 2015 Jan;8(1):14-8. doi: 10.1016/S1995-7645(14)60180-2.
To investigate the susceptibility to temephos, permethrin and deltamethrin of Aedes aegypti (Ae. aegypti), collected from areas with high incidence of dengue hemorrhagic fever cases in Phitsanulok Province, Thailand.
The F1 progenies of Ae. aegypti colony, originated from five sub-districts including Aranyik, Hua Ro, Nai Muang, Ban Krang and Tha Pho, were used in the bioassays following the procedures of World Health Organization. For larval bioassay, the late third or early fourth-instar larvae were tested with different concentrations of temephos. For adult bioassay, the females were exposed to 0.75 % permethrin or 0.05% deltamethrin. LC50 value and mortality rate were analyzed to compare the insecticide susceptibility of the larvae and the adults in each area, respectively.
The LC50 value of temephos for the larvae from Aranyik, Hua Ro, Nai Muang, Ban Krang and Tha Pho sub-districts was 0.017, 0.017, 0.026, 0.061, and 0.113 ppm, respectively. For permethrin, the highest mortality rate (86.84%) was found in the mosquitoes from Aranyik but the others were more resistant with the lower mortality rates (16.00-42.67%). The adult mortality rates after exposing to deltamethrin were higher (82.34-98.67%) in all areas.
Ae. aegypti larvae were still susceptible to temephos. Conversely, most tested adults tended to resist the permethrin and deltamethrin.
调查从泰国彭世洛府登革出血热病例高发地区采集的埃及伊蚊对双硫磷、氯菊酯和溴氰菊酯的敏感性。
按照世界卫生组织的程序，对源自阿兰尼克、华罗、乃孟、班克朗和他波五个分区的埃及伊蚊群体的F1代子代进行生物测定。对于幼虫生物测定，用不同浓度的双硫磷测试三龄晚期或四龄早期幼虫。对于成虫生物测定，将雌蚊暴露于0.75%的氯菊酯或0.05%的溴氰菊酯中。分析半数致死浓度（LC50）值和死亡率，以比较每个地区幼虫和成虫对杀虫剂的敏感性。
阿兰尼克、华罗、乃孟、班克朗和他波分区幼虫的双硫磷LC50值分别为0.017、0.017、0.026、0.061和0.113 ppm。对于氯菊酯，在来自阿兰尼克的蚊子中发现最高死亡率（86.84%），但其他蚊子更具抗性，死亡率较低（16.00 - 42.67%）。在所有地区，暴露于溴氰菊酯后的成虫死亡率较高（82.34 - 98.67%）。
埃及伊蚊幼虫对双硫磷仍敏感。相反，大多数测试的成虫倾向于对氯菊酯和溴氰菊酯产生抗性。
