Ban Sayaka, Azuma Yuta, Sato Hiroki, Suzuki Ken-Ichiro, Nakagiri Akira
NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba, 292-0818, Japan.
Japanese Society of Cordyceps Research, Kakinoki, Kakinoki-mura, Yoshika-cho, Kanoashi-gun, Shimane, 699-5301, Japan.
Int J Syst Evol Microbiol. 2015 Aug;65(8):2459-2465. doi: 10.1099/ijs.0.000284. Epub 2015 Apr 24.
The entomogenous anamorphic fungus Isaria takamizusanensis has not been resolved clearly in its teleomorphic state. We succeeded in inducing ascostroma formation by incubating conidiomata of I. takamizusanensis on cicada adults in a moist chamber. We observed the ascostroma and conducted a phylogenetic analysis based on ITS rDNA and EF-1α genes. The morphology of the ascostroma was identical to that of Cordyceps ryogamimontana. In the phylogenetic tree inferred from EF-1α, the isolate from the partspores grouped with nine strains derived from conidia of I. takamizusanensis, which was distinct from a clade including Purpureocillium lilacinum. Moreover, a conidial structure identical to that of I. takamizusanensis was rediscovered on the holotype specimen of C. ryogamimontana. As a result, we propose a new name, Purpureocillium takamizusanense, which is a combination of the teleomorph-anamorph connection of C. ryogamimontana-I. takamizusanensis, in accordance with the 'one fungus, one name' concept of the International Code of Nomenclature for Algae, Fungi, and Plants (ICN).
虫生无性型真菌高见岳虫草菌（Isaria takamizusanensis）的有性型状态尚未得到明确解析。我们通过将高见岳虫草菌的分生孢子器在潮湿环境中于蝉成虫上培养，成功诱导出子座形成。我们观察了子座，并基于ITS rDNA和EF-1α基因进行了系统发育分析。子座的形态与山梨虫草（Cordyceps ryogamimontana）的相同。在基于EF-1α推断的系统发育树中，来自分生子的分离株与源自高见岳虫草菌分生孢子的九个菌株聚在一起，这与包括淡紫拟青霉（Purpureocillium lilacinum）的一个分支不同。此外，在山梨虫草的模式标本上重新发现了与高见岳虫草菌相同的分生孢子结构。结果，我们根据《藻类、真菌和植物国际命名法规》（ICN）的“一种真菌，一个名称”概念，提出了一个新名称，即淡紫高见岳拟青霉（Purpureocillium takamizusanense），它是山梨虫草 - 高见岳虫草菌有性型 - 无性型的组合。
