Pfaller M, Hollis R, Johnson W, Massanari R M, Helms C, Wenzel R, Hall N, Moyer N, Joly J
Veterans Administration Medical Center, Iowa City, Iowa.
Diagn Microbiol Infect Dis. 1989 Jul-Aug;12(4):295-302. doi: 10.1016/0732-8893(89)90093-x.
We applied monoclonal antibody typing and restriction endonuclease analysis of plasmid DNA to study 28 clinical and 35 environmental (potable water) isolates of Legionella pneumophila serogroup 1 from three hospitals in Iowa between 1981 and 1986. Monoclonal antibody typing employed a panel of seven antibodies and delineated eight different subtypes. Plasmids were present in 57% of the isolates including 12 of 28 (43%) clinical and 25 of 35 (69%) potable water isolates. The plasmids ranged in size from 28 to 98 kilobase pairs and comprised eight distinct subtypes by restriction endonuclease analysis with Eco RI. Combination of monoclonal antibody and restriction endonuclease subtyping (composite subtyping) revealed 19 different composite subtypes of Legionella pneumophila serogroup 1. The most common composite subtype, 09:04, comprised 29% (18 of 63) of the isolates and was only found in clinical and potable water samples from a single pavilion in hospital A during an outbreak of Legionella pneumophila serogroup 1 pneumonia. Aside from this cluster the diversity of composite subtypes of Legionella pneumophila serogroup 1 observed in clinical and potable water sources over the 5-year period was striking. The combination of monoclonal antibody and restriction endonuclease typing resulted in improved strain delineation and a more useful use of epidemiologic markers for Legionella pneumophila serogroup 1.
我们应用单克隆抗体分型和质粒DNA的限制性内切酶分析，对1981年至1986年间从爱荷华州三家医院分离出的28株临床菌株和35株环境（饮用水）嗜肺军团菌血清1型菌株进行了研究。单克隆抗体分型使用一组七种抗体，划分出八种不同的亚型。57%的菌株含有质粒，其中包括28株临床菌株中的12株（43%）和35株饮用水菌株中的25株（69%）。质粒大小从28到98千碱基对不等，通过用Eco RI进行限制性内切酶分析，可分为八种不同的亚型。单克隆抗体和限制性内切酶亚型分型相结合（复合分型），揭示了嗜肺军团菌血清1型的19种不同复合亚型。最常见的复合亚型09:04，占分离菌株的29%（63株中的18株），仅在医院A一个病房爆发嗜肺军团菌血清1型肺炎期间的临床和饮用水样本中发现。除了这一聚集情况外，在5年期间临床和饮用水源中观察到的嗜肺军团菌血清1型复合亚型的多样性非常显著。单克隆抗体和限制性内切酶分型相结合，提高了菌株的划分能力，并使嗜肺军团菌血清1型的流行病学标记物得到更有效的应用。
