Maher W E, Para M F, Plouffe J F
Department of Medicine, Ohio State University College of Medicine, Columbus 43210-1228.
J Clin Microbiol. 1987 Dec;25(12):2281-4. doi: 10.1128/jcm.25.12.2281-2284.1987.
A group of environmental and clinical Legionella pneumophila serogroup 1 isolates was subtyped by monoclonal antibody dot immunoblotting and plasmid analysis. Monoclonal antibody analysis defined seven subtypes within three major groups. Plasmid analysis (including restriction endonuclease digestion) revealed 10 subtypes. By combining plasmid and monoclonal techniques, all 16 strains were shown to be distinct. Plasmid profiles and monoclonal antibody reactivities of selected strains were stable despite serial passage (greater than 100 times). No plasmid-associated antigen was defined by this panel of monoclonal antibodies. The observed dissociation of plasmid profiles and monoclonal antibody reactivity patterns suggests that accurate epidemiologic typing of L. pneumophila serogroup 1 strains will require use of both techniques.
一组环境和临床来源的嗜肺军团菌血清1型分离株通过单克隆抗体斑点免疫印迹法和质粒分析进行亚型分型。单克隆抗体分析在三个主要组内确定了七个亚型。质粒分析（包括限制性内切酶消化）显示有10个亚型。通过结合质粒和单克隆技术，所有16株菌株均显示出不同。尽管经过连续传代（超过100次），所选菌株的质粒图谱和单克隆抗体反应性仍保持稳定。这组单克隆抗体未确定与质粒相关的抗原。观察到的质粒图谱和单克隆抗体反应模式的分离表明，对嗜肺军团菌血清1型菌株进行准确的流行病学分型需要同时使用这两种技术。
