一种全血中谷胱甘肽的简易检测方法。
A simple assay for glutathione in whole blood.
作者信息
Hakuna Lovemore, Doughan Brandon, Escobedo Jorge O, Strongin Robert M
机构信息
Department of Chemistry, Portland State University, Portland, OR 97201, USA.
出版信息
Analyst. 2015 May 21;140(10):3339-42. doi: 10.1039/c5an00345h. Epub 2015 Apr 27.
Abstract
A method for detecting glutathione selectively in whole blood deposited on filter paper is described. GSH is fractionated from proteins, hemoglobin and other potentially interfering components and determined using a resorufin-acrylate fluorescent probe. The relative standard deviation is lower than 5% (n = 5). Recoveries of GSH from whole blood are between 94% and 108.6%.
摘要
描述了一种用于选择性检测沉积在滤纸上的全血中谷胱甘肽的方法。谷胱甘肽（GSH）从蛋白质、血红蛋白和其他潜在干扰成分中分离出来，并使用试卤灵丙烯酸酯荧光探针进行测定。相对标准偏差低于5%（n = 5）。全血中谷胱甘肽的回收率在94%至108.6%之间。
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