Li Pengchao, Tao Jun, Wei Dandan, Yang Xiao, Lu Zhaoguang, Deng Xiaheng, Cheng Yiong, Gu Jinbao, Yang Xuejian, Wang Zengjun, Lu Qiang, Wang Junsong, Yin Changjun
Department of Urology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People's Republic of China.
Center for Molecular Metabolism, Nanjing University of Science and Technology, Nanjing, 210094, People's Republic of China.
Tumour Biol. 2015 Sep;36(10):7531-7. doi: 10.1007/s13277-015-3482-3. Epub 2015 Apr 28.
To find potential serum biomarkers for upper tract urothelial carcinomas (UTUCs) via (1)H nuclear magnetic resonance ((1)H NMR)-based metabolomic analysis. Serum specimens collected from 34 healthy individuals and 39 patients with UTUCs were subjected to (1)H NMR-based metabolomic analysis. Principal component and orthogonal partial least squares discriminant analyses were used to analyse the data. Compared with serum samples from healthy subjects, samples from UTUC patients showed elevated levels of lactate and creatinine as well as decreased levels of glucose, glutamine and taurine. Serum low-density lipoprotein/very low-density lipoprotein, valine and glycoprotein levels showed decreasing trends whereas serum polyunsaturated fatty acids and 3,7-dimethyluric acid level presented increasing trends in UTUC patients. (1)H NMR-based metabolomic analysis of serum enhances the current understanding of the mechanisms involved in UTUC development. The present analysis may be a valuable tool for UTUC detection.
通过基于氢核磁共振（¹H NMR）的代谢组学分析寻找上尿路尿路上皮癌（UTUC）的潜在血清生物标志物。收集34名健康个体和39名UTUC患者的血清样本，进行基于¹H NMR的代谢组学分析。采用主成分分析和正交偏最小二乘法判别分析对数据进行分析。与健康受试者的血清样本相比，UTUC患者样本中的乳酸和肌酐水平升高，而葡萄糖、谷氨酰胺和牛磺酸水平降低。UTUC患者的血清低密度脂蛋白/极低密度脂蛋白、缬氨酸和糖蛋白水平呈下降趋势，而血清多不饱和脂肪酸和3,7-二甲基尿酸水平呈上升趋势。基于¹H NMR的血清代谢组学分析加深了目前对UTUC发生机制的理解。本分析可能是UTUC检测的一个有价值的工具。
