Jayanthi M, Garg Satish K, Yadav Prashant, Bhatia A K, Goel Anjana
Department of Pharmacology and Toxicology, U.P. Pandit Deen Dayal Upadhayaya Veterinary and Animal Sciences University, Mathura, Uttar Pradesh, India.
Department of Microbiology, U.P. Pandit Deen Dayal Upadhayaya Veterinary and Animal Sciences University, Mathura, Uttar Pradesh, India.
Indian J Pharmacol. 2015 Sep-Oct;47(5):518-23. doi: 10.4103/0253-7613.165199.
The present study was undertaken to unravel the newer marker phytoconstituents in methanolic extract of Moringa oleifera leaves (MOLE) and evaluation of its immunomodulatory and splenocytes proliferation potential in rats.
Hot methanolic extract of MOLE was subjected to gas chromatography-mass spectrometry (GC-MS) analysis. Immunomodulatory potential was studied in four groups of rats following administration of MOLE at 62.5 and 125 mg/kg for 21 days, followed by immunization with Salmonella typhimurium "O" antigen and antibody titer determined using indirect enzyme-linked immunosorbent assay kit. Total lymphocytes and T- and B-lymphocytes count were determined in control and after MOLE administration (62.5 and 125 mg/kg) to rats for 42 days. Splenocytes (2 × 10(6) spleen cells/ml) from MOLE treated rats were harvested and stimulated using concanavalin A and optical density (OD) and stimulation index were determined. Splenocytes from healthy control rats were also collected and treated in vitro with different concentrations of MOLE (5, 10, 25, 50, and 100 µg/ml) and concanavalin A to determine effect of MOLE on OD and stimulation index.
GC-MS analysis revealed presence of 9,12,15-octadecatrienoic acid ethyl ester, 6-octadecenoic acid, cis-vaccenic acid and 2-octyl-cyclopropaneoctanal in MOLE. MOLE at 125 mg/kg increased the antibody titer by 50%. Although there was slight decline in lymphocytes count (total, B- and T-lymphocytes) in MOLE treated rats, percentage of T-lymphocytes was increased nonsignificantly. Ex vivo and in vitro studies revealed marked increase in OD and stimulation index indicating MOLE-induced splenocytes proliferation.
GC-MS study revealed four new compounds in MOLE apart from promising its immunomodulatory potential based on humoral immune response, percentage increase in T-lymphocytes count, and induction of splenocytes proliferation.
本研究旨在揭示辣木树叶甲醇提取物（MOLE）中更新的标志性植物成分，并评估其对大鼠的免疫调节和脾细胞增殖潜力。
对MOLE的热甲醇提取物进行气相色谱 - 质谱（GC - MS）分析。将四组大鼠给予62.5和125 mg/kg的MOLE，持续21天，随后用鼠伤寒沙门氏菌“O”抗原进行免疫，并使用间接酶联免疫吸附测定试剂盒测定抗体效价，研究其免疫调节潜力。在对照组以及给予大鼠MOLE（62.5和125 mg/kg）42天后，测定总淋巴细胞以及T淋巴细胞和B淋巴细胞的数量。收集经MOLE处理的大鼠的脾细胞（2×10⁶脾细胞/ml），用刀豆球蛋白A刺激，并测定光密度（OD）和刺激指数。还收集健康对照大鼠的脾细胞，用不同浓度的MOLE（5、10、25、50和100 μg/ml）和刀豆球蛋白A进行体外处理，以确定MOLE对OD和刺激指数的影响。
GC - MS分析显示MOLE中存在9,12,15 - 十八碳三烯酸乙酯、6 - 十八碳烯酸、顺式 - vaccenic酸和2 - 辛基 - 环丙烷辛醛。125 mg/kg的MOLE使抗体效价提高了50%。虽然经MOLE处理的大鼠的淋巴细胞数量（总淋巴细胞、B淋巴细胞和T淋巴细胞）略有下降，但T淋巴细胞的百分比无显著增加。体内外研究均显示OD和刺激指数显著增加，表明MOLE诱导脾细胞增殖。
GC - MS研究揭示了MOLE中的四种新化合物，此外，基于体液免疫反应、T淋巴细胞数量的百分比增加以及脾细胞增殖的诱导，其具有良好的免疫调节潜力。
