Ahmed A M Abu, Rahman Md Atiar
Department of Biochemistry and Molecular Biology, University of Chittagong, Chittagong, Bangladesh.
Department of Genetic Engineering and Biotechnology, University of Chittagong, Chittagong, Bangladesh.
J Adv Vet Anim Res. 2021 Sep 20;8(3):479-488. doi: 10.5455/javar.2021.h537. eCollection 2021 Sep.
This research investigated the immunomodulatory potentials of two medicinally important wild epiphytic Bangladeshi orchids and using Swiss albino mice.
Orchid extracts were prepared using a cold methanol extraction procedure. To assess the immunomodulatory action, Swiss albino mice of either sex weighing 25-35 gm were divided into five groups each with six animals. Sheep red blood cells (SRBC) of 0.5 × 10 cells/ml were used to immunize all mice on the 7th day, and a booster dose of the same quantity of SRBC was given on the 11th day of the experiment. After 14 days of oral treatment with 100 and 200 mg/kg bw of orchid extract, the mice were sacrificed to collect serum and organs. Hematological assays, delayed type of hypersensitivity assays, phagocytic index (PI), and histopathological investigations were used to assess immunomodulatory efficacy.
The body weight changes of the experimental animals were considerably greater at 100 mg/kg bw than at a higher dose (200 mg/kg bw). There was a substantial improvement of relative organ weights of the thymus and spleen at the low dose, but no effect on kidney weights was evident. The liver weight increased significantly ( < 0.05) at both doses. Total neutrophil, leukocyte, and lymphocyte counts, hemoglobin percentage, delayed hypersensitivity reaction, and PI were all significantly ( < 0.05) increased in mice receiving the lower dose. In contrast to the control group, the higher dose reduced immunological response, suggesting the negative influence of a higher dose of extracts on the immune reaction.
The results demonstrate that orchid extracts can potentially modulate the innate immune system in the experimental animal.
本研究利用瑞士白化小鼠，调查了两种具有重要药用价值的孟加拉野生附生兰花的免疫调节潜力。
采用冷甲醇提取法制备兰花提取物。为评估免疫调节作用，将体重25 - 35克的雌雄瑞士白化小鼠分为五组，每组六只。在实验第7天，用0.5×10个细胞/毫升的绵羊红细胞（SRBC）对所有小鼠进行免疫，并在实验第11天给予相同数量的SRBC加强剂量。在用100和200毫克/千克体重的兰花提取物进行口服治疗14天后，处死小鼠以收集血清和器官。通过血液学检测、迟发型超敏反应检测、吞噬指数（PI）和组织病理学研究来评估免疫调节功效。
实验动物在100毫克/千克体重剂量下的体重变化比高剂量（200毫克/千克体重）时显著更大。低剂量时胸腺和脾脏的相对器官重量有显著改善，但对肾脏重量没有明显影响。两种剂量下肝脏重量均显著增加（P<0.05）。接受较低剂量的小鼠中，总中性粒细胞、白细胞和淋巴细胞计数、血红蛋白百分比、迟发型超敏反应和PI均显著（P<0.05）增加。与对照组相比，高剂量降低了免疫反应，表明高剂量提取物对免疫反应有负面影响。
结果表明兰花提取物可能对实验动物的先天免疫系统具有调节作用。
